Functional SNPs associated with the fat profile of pork loin intramuscular fat

• Grant number: 22/08142-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2023
• End date: August 31, 2024
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Aline Silva Mello Cesar
• Grantee: Mariah Castro Durval
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 17/25180-2 - Effect of dietary oleic acid on fatty acid composition and inflammatory response profile in different tissues of pigs, AP.JP
• Associated scholarship(s): 23/07931-1 - Identifying candidate functional SNPs associated with the fatty acid profile of pork loin intramuscular fat, BE.EP.MS

Abstract

Pork meat stands out as one of the most consumed proteins worldwide. Due to its economic and health importance, being used as a model animal in human research, this species, Sus scrofa, was chosen for this study. This meat has a high nutritional value. Moreover, it's a great supplier of unsaturated fatty acids since humans need to include them in their diet. In this experiment, information used is from seventy-two Large White males, immunocastrated and homozygous-negative halothane (NN), slaughtered around 130 kg.The animals obtained four different diets during their growth. Respectively, 1.5% of degummed soybean oil, basal with 3% of soybean oil, basal with 3% of canola oil, and basal with 3% of soybean oil. From fish: oil from sardines (Cetengraulis edentulus). Immediately after slaughter, we collect the tissue samples for mRNA extraction and sequencing. The objective of the present project is to identify functional polymorphisms of the SNP type and eQTLs associated with the characteristics of fatty acid profilefrom large-scale genotyping data and the muscle transcriptome of Large White swine fed a diet with the different acid profiles of fat. For this, the association study approach by linear model of genotypic data (SNP chip - Porcine GGP 50k) and gene expression (RNA-Seq) will be used.