In vitro evaluation of modulations of equine dermal scaffolds for use in regenerative medicine

• Grant number: 23/05221-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2023
• End date: May 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Maria Angelica Miglino
• Grantee: Maria Laura dos Reis Ferre Pereira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Tissue Bioengineering aims at the production of biomaterials for use in regenerative medicine, such as scaffolds and biogels. The produced biomaterials can be applied to replace an injured tissue or organ, serve as a substrate for cell culture or as a supplement to culture media. For the application of these biomaterials in vivo, several actions and in vitro tests are necessary, such as sterility, cytotoxicity and cell viability tests. It is common to observe skin lesions in all species, having no relation to age or sex. In animal species, fighting and agitated behavior often leads to the development of skin lesions, as well as burns and carcinomas. Thus, tissue bioengineering has shown promising results in the development of acellular biological scaffolds through the decellularization technique. After decellularization, the scaffold can be applied in different ways, including cells from the animal that will receive the graft. Thus, the use of these scaffolds for implantation in skin wounds reduces the risk of rejection, improving the response to the implant. Thus, the objective of this work is to promote the in vitro evaluation of modulations of decellularized scaffolds of equine dermis through cell-scaffold interaction with histological and immunological tests, scanning electron microscopy and cell metabolism. For this purpose, commercial 3T3 cells (murine fibroblasts) and equine fibroblasts from primary culture will be used. With this, it is expected to contribute to the development of a biomaterial for dermal implants in veterinary medicine, thus avoiding double surgical fields and the need for compatible donors.