Evaluation of Salmonella Gallinarum behaviour strain 287/91 containing both cobS and cbiA defective genes during infection of chicken primary macrophage.

• Grant number: 23/04863-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2023
• End date: February 28, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Angelo Berchieri Junior
• Grantee: Isabella Cardeal Campos
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Fowl typhoid is an important disease for the poultry industry, due to the damage caused by the highest chicken mortality, which can reach 80% or more. Among the main biosecurity measures are the correct management in the poultry house and the adoption of vaccines. The present study aims to continue the previous work of this research group with a mutant strain of Salmonella Gallinarum containing the deletion of the cobS and cbiA genes, a potential candidate vaccine. The objective is to infect chicken primary macrophages, the main cells involved in the pathogenesis of fowl typhoid, comparing the capture and survival with the wild strain. Furthermore, the differential gene expression of both strains will be verified, providing further information on the behaviour of these bacteria during infection.