Dentin treatment and interaction with bioceramic sealers after bioglass application

• Grant number: 22/15839-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2023
• End date: April 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Giovanna da Cunha Mendonça
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Bioceramic endodontic sealers deserve attention due to their biological properties and interaction with dentin. Bioglass particles, considered a type of bioactive ceramic composed of silicon oxide, calcium pentoxide, sodium oxide, sodium pentoxide can be used to promote greater bioactivity and adhesion with dentin. The Laboratory of Vitreous Materials (LaMaV - UFSCar) developed a bioglass, F18, with high bioactivity. This study aims to develop protocols for the treatment of dentin, prior to the use of powder-liquid or ready-to-use endodontic sealers. The effect of dentin treatment (bioglass suspension at a concentration of 2.5%; 5.0% and 10.0% and distilled water as a control) on dentin and cement/dentin interface will be evaluated. The bond strength of the filling material with the dentin will be evaluated by using the push-out test. The test will be performed using cylindrical bovine dentin tubes with an internal diameter of 1.3 mm, wall thickness of approximately 1.0 mm. The specimens will be subdivided into groups according to the treatment of dentin and endodontic sealer: Bio-C Sealer (Angelus, Brazil), BioRoot RCS (Septodont, France) or AH Plus (Dentsply, Germany). The evaluation of the bond strength (push-out) will be carried out using an Emic DL 2000 testing machine with a 1 kN load cell operating at a constant speed of 0.5 mm/min. The data obtained for each evaluation will be submitted to the appropriate statistical tests, with a significance level of 5%.