Advanced biological seal on drug delivery system through MAA-based film construction. A complex strategy to fight disease and optimizing soft tissue integration

• Grant number: 23/11031-6
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): September 01, 2023
• Effective date (End): August 31, 2025
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Érica Dorigatti de Avila
• Grantee: Daniela Moreira Cunha
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated research grant: 18/20719-3 - A novel antimicrobial drug-delivery coating for percutaneous implant devices, AP.JP
• Associated scholarship(s): 24/12622-0 - Advanced biological/nonresponsive film (ABNrF) for soft tissue integration, BE.EP.MS

Abstract

The feasibility of our proposed approaches is based on our previous studies (de Avila et al., 2019; Verza et al. 2021), including our preliminary outcomes detailed in the scientific report. We have already confirmed the succeeded multilayer system through both polyelectrolytes: poly(acrylic acid) (PAA) and poly(l-lysine) (PLL), in ten double layers (de Avila et al., 2019). We have also validated the drug incorporation method into the layer-by-layer system (LbL), through the synthesis of a hydrophilic drug with anionic beta cyclodextrin (²-CD) *(Verza et al., 2021). Furthermore, we have created earlier an acidic pH sensible film with methacrylic acid (MAA)-based vascular regenerative to cover [PAA/PLL]10/TC/anionic ²-CD and to promote vascularization and subsequent wound healing. Up to now, we obtained promisor results in terms of not injuring the cells and protecting the drug within the system for longer. However, we only got the desired effect on human cells after including the wash steps of the MAA-based film. the remaining amount of drug incorporated into the system is sufficient to act against all salivary bacteria, the main disadvantage of this process is the early loss of drug from the system. Here, we will focus on creating a strategy to remove the acidic property of MAA material powder before developing the film. Moreover, the tailor-innovative film will be also constructed to improve the sealing stability between component and soft tissue through specific components modulation from hemidesmossomes. A detailed multilayer coating characterization will be performed by different microscopy and spectroscopy approaches to probe physical and chemical properties. Coating stability of MAA-based film on [PAA/PLL]10/TC/anionic ²-CD system will be confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. Moreover, the molecular and cellular mechanisms of interaction in the presence of MAA-based film will be investigate to improve the therapeutic effect of layer-by-layer coating system.