Pilot study on cellular events involved in the immunomodulatory effect of murine IgG antibodies on thymic differentiation of TCD4, TCD8, gamma-deltaT, and B cells

• Grant number: 23/16782-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2024
• End date: June 30, 2025
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Jefferson Russo Victor
• Grantee: Lais Alves do Nascimento
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 21/08225-8 - The Hooks without bait theory: mechanisms involved in the immunomodulatory effect of IgG on the thymic modulation of TCD4, TCD8, gdT, iNKT MAIT and B cells, focus on atopic diseases and COVID-19, AP.JP

Abstract

IgG antibodies from allergen-tolerant mice can modulate the activation of B lymphocytes and the production of cytokines by peripheral T lymphocytes in vivo. They can induce the differentiation of peripheral regulatory B lymphocytes, producing IL-10 (B10), which exerts an inhibitory role in developing allergic lung inflammation. Furthermore, it has also been demonstrated that these antibodies can induce a population of murine peripheral regulatory B lymphocytes characterized by the production of IL-17 (B17 or Br17) and that the maturation of B10 lymphocytes, at least when mediated by IgG from tolerant animals, may occur preferentially in the thymus. In the context of T lymphocytes, a recent study showed that IgG from allergen-tolerant animals can inhibit the thymic maturation of IL-17 (Th17)-producing T lymphocytes in vivo, which can amplify the pulmonary allergic response. The general context of the proposal to which this request is linked includes several approaches in which the effect of the repertoire of IgG antibodies produced in humans with different established pathological processes will be evaluated on the functional modulation of thymic and peripheral T and B lymphocytes, however, still There is no description of similar mechanisms in the murine context in the literature. Based on this, it is necessary to carry out a pilot study where it is possible to comparatively evaluate the effects mediated by different repertoires of murine IgG on thymic lymphocytes in vitro, in parameters similar to those adopted for analyses with human samples yielding a comparative analysis of the results.