Fabrication of multiplex electrochemical (bio)sensors by CO2 laser engraving aiming the development of wearable devices.

• Grant number: 23/12589-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2024
• End date: January 31, 2026
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Thiago Regis Longo Cesar da Paixão
• Grantee: Lucas Felipe de Lima
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Wearable devices are a promising alternative with regard to continuous monitoring and clinical diagnosis of diseases in affected individuals, providing analyzes directly on the human body in real time, without the need for sample preparation or laboratory infrastructure. Most of these devices focus mainly on applications aimed at the health area, to monitor various pathogens and diseases of clinical and hospital interest, which have the potential to assist medical work in the detection and monitoring of contagious diseases, for example. The development of easy-to-handle electrochemical (bio)sensors with rapid clinical diagnosis can be an important tool for monitoring biomarkers or chemical species of interest, qualitatively and/or quantitatively, from direct contact with the surface or with the biofluid of interest. Thus, this research project aims to develop an array of wearable electrochemical (bio)sensors on a paper platform, functionalize them (in-situ and ex-situ) with different nanomaterials and enzymes for qualitative detection (screening) and quantitative analysis of molecules present in human sweat that are attractive to the Anopheles gambiae mosquito that causes Malaria. The array of electrochemical (bio)sensors will be fabricated by surface carbonization induced by CO2 laser radiation on paper substrates chemically treated with flame retardant. This process aims to produce materials with excellent electrochemical performance, in an affordable way, without or with minimal consumption of chemical reagents, reducing the toxicity of the material for wearable applications, as well as increasing its disposability and scalable manufacturing.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; RODRIGUES, JESSICA DE SOUZA; DE LIMA, LUCAS FELIPE; PAIXAO, THIAGO REGIS LONGO CESAR; FRACETO, LEONARDO FERNANDES; JANEGITZ, BRUNO CAMPOS. Advancements in disposable electrochemical systems for sustainable agriculture monitoring: Trends, gaps, and applied examples. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 180, p. 14-pg., 2024-09-13. (17/21004-5, 22/03399-0, 21/10639-5, 19/04758-1, 19/23342-0, 23/12589-0, 23/00246-1, 23/06793-4, 19/23177-0, 22/00509-0, 23/00335-4)

BOTTELLI, ELISA D.; DE LIMA, LUCAS F.; PAIXAO, THIAGO R. L. C.; DE ARAUJO, WILLIAM R.. Laser-scribed graphene toward scalable fabrication of electrochemical paper-based devices for lidocaine detection in forensic and pharmaceutical samples. Electrochimica Acta, v. 507, p. 10-pg., 2024-10-07. (23/12589-0, 18/08782-1, 23/00246-1, 22/03250-7)

DE LIMA, LUCAS FELIPE; FERREIRA, ANDRE LOPES; DO NASCIMENTO, GUIDA HELLEN MOTA; CARDOSO, LUANA PEREIRA; DE JESUS, MARCELO BISPO; DE ARAUJO, WILLIAM REIS. Tongue depressor (bio)sensors: A fast decentralized self-testing of salivary biomarkers for personalized medicine. CHEMICAL ENGINEERING JOURNAL, v. 494, p. 13-pg., 2024-06-18. (22/09892-0, 23/03483-4, 23/05204-5, 23/12589-0, 23/06143-0, 23/00246-1, 18/08782-1, 22/03250-7)