Overexpression of new target gene associated with fish myoblast fusion and/or proliferation in indeterminate muscle growth pattern

• Grant number: 24/00192-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 06, 2024
• End date: December 05, 2024
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maeli Dal Pai
• Grantee: Erika Stefani Perez
• Supervisor: Daniel Garcia de La Serrana Castillo
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Universitat de Barcelona (UB), Spain
• Associated to the scholarship: 21/13905-8 - Transcriptomic and functional analysis as strategies for understanding the determinate and indeterminate muscle growth patterns of fish, BP.DR

Abstract

Muscle growth in fish occurs by hypertrophy and hyperplasia. The hyperplasia can be classified in two types: mosaic and stratified. Fish with mosaic hyperplasia continuous throughout life have indeterminate growth, such as Piaractus mesopotamicus (pacu). Fish with mosaic hyperplasia more restricted to the first days after hatching, have determined growth, such as Danio rerio (zebrafish). Numerous studies carried out over the years have contributed to knowledge about muscle growth, however, this process is not fully understood. In this sense, omics analysis are efficient tools that provide a global view of genes that coordinate complex processes, such as muscle growth. Furthermore, the use of omic data from public databases associated with functional assays can provide new information about biological networks involved with phenotype control and muscle growth. This project aims to validate noc3l or wasla genes obtained previously from pacu and zebrafish transcriptomes analysis. For this proposal, we will do the zebrafish myoblast culture and will transfect, using magnetoporation, noc3l/wasla genes to overexpress them. Considering the robust methodology that will be used, it is expected that the knowledge generated by this project can complement the results being obtained in the doctoral project financed by FAPESP (2021/13905-8). In addition, it can contribute with the development of innovation projects that are of great interest to the sector productive. Thinking in the technological learning, the magnetotransfection could be applied in our laboratory in Brazil using other species of fish.