Intersection between stress responses in bacteria

• Grant number: 24/03640-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2024
• End date: April 30, 2026
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Rodrigo da Silva Galhardo
• Grantee: Valdir Cristovao Barth Junior
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 21/10577-0 - Biology of Bacteria and Bacteriophages Research Center, AP.CEPID

Abstract

Both ppGpp signaling and the SOS response, induced by DNA damage, are universal in bacteria and central to survival in the environment, pathogenesis and antimicrobial resistance. The project aims to use bacterial genetics, molecular biology and biochemistry techniques to understand the interaction between these two responses in the opportunistic pathogen Pseudomonas aeruginosa and the model bacterium Bacillus subtilis. In this project we will analyze whether the induction of one response is dependent or influenced by the other and how this intersection occurs, as well as the biological consequences of this interaction. We will also investigate the yet poorly explored role of ppGpp in the modulation of transcription under normal growth conditions.