Validation of an analytical method for subsequent in vitro metabolism study of the sunscreen octocrylene by human liver microsomes.

• Grant number: 24/05759-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2024
• End date: May 31, 2026
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Anderson Rodrigo Moraes de Oliveira
• Grantee: Vinicius Roberto Poli
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

UV filters, present in sunscreen lotions and creams, are widely used in the population's daily lives, as they can absorb the ultraviolet radiation emitted by the sun and prevent human skin from possible damage caused by excessive and prolonged exposure. Among the main substances present in sunscreens, with the function of UV filter, the project focuses on the study of octocrylene, a UV filter and stabilizer of another widely used compound, avobenzone. Due to the fact that sunscreens are used worldwide, several organic active ingredients present in lotions have already been found in the waters of rivers, lakes and oceans, even after passing through treatment plants, thus being potential contaminating agents for the environment. Additionally, some UV filters have been found in human biological material such as urine and breast milk. On the other hand, the effect of sunscreens on cytochrome P450 (CYP450) enzymes is still poorly described in the literature. CYP450 enzymes are involved in the metabolism of endogenous compounds and are mainly responsible for drug metabolism. Therefore, changes in the activity of these enzymes can lead to changes in the pharmacokinetics of drugs. Therefore, due to the high exposure of humans to UV filters as well as studies reporting its presence in biological material, this project aims to carry out an in vitro metabolism study of octocrylene using human liver microsomes. To this end, an analytical method will be previously validated and subsequently the enzymatic parameters, KM and VMAX, will be determined, so that the CLH can be obtained and finally the in vitro-in vivo extrapolation can be carried out.