Development and validation of chromatographic and electrophoretic methods for subsequent application in studies of in vitro metabolism and biotransformation - phase 2

• Grant number: 13/17658-9
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2013
• End date: November 30, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Anderson Rodrigo Moraes de Oliveira
• Grantee: Anderson Rodrigo Moraes de Oliveira
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto
• Associated researchers: Mônica Tallarico Pupo ; Norberto Peporine Lopes

Abstract

The quantification of analytes present in biological matrices requires the development of analytical methods that should be precise, accurate and reproducible under several conditions of analysis. Chromatographic and electrophoretic techniques (capillary electrophoresis) are techniques widely used for these purposes. These techniques have several advantages such as: simplicity, speed of the analysis and, great versatility. Our research group has been working on the development and validation of chromatographic and electrophoretic methods to be further applied in biotransformation studies and in "in vitro" metabolism studies. These studies follow two distinct lines: (1) In vitro metabolism studies of potentially therapeutic compounds using liver microsomes and (2) use of fungi in the biotransformation of drugs, whose metabolism yields in chiral and pharmacologically active metabolites. In this context, it will be developed and validated bioanalytical methods for these purposes. This project aims to continue our research project, which has been supported by FAPESP (grant numbers 2009/06132-0 and 2011/17508-1). The last one will expire on November 30, 2013. It also seeks to solidify a new line of research in the Chemistry Department of Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. In addition, this project aims in the formation of graduate students in master's and doctoral modalities. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, BRUNO ALVES; MORAES DE OLIVEIRA, ANDERSON RODRIGO; PAZIN, MURILO; DORTA, DANIEL JUNQUEIRA; RODRIGUES, ANDRESA PIACEZZI NASCIMENTO; BERRETTA, ANDRESA APARECIDA; PETI, ANA PAULA FERRANTI; BERALDO DE MORAES, LUIZ ALBERTO; LOPES, NORBERTO PEPORINE; POSPISIL, STANISLAV; et al. Jacobsen Catalyst as a Cytochrome P450 Biomimetic Model for the Metabolism of Monensin A. BIOMED RESEARCH INTERNATIONAL, 2014. (13/17658-9, 11/05800-0)

BOCATO, MARIANA ZUCCHERATO; BORTOLETO, MARCELA ARMELIM; PUPO, MONICA TALLARICO; MORAES DE OLIVEIRA, ANDERSON RODRIGO. A new enantioselective CE method for determination of oxcarbazepine and licarbazepine after fungal biotransformation. ELECTROPHORESIS, v. 35, n. 19, SI, p. 2877-2884, OCT 2014. (13/17658-9)

BARTH, THIAGO; HILARIO, VIVIANE C.; ROCHA, BRUNO A.; FURTADO, NIEGE A. J. C.; PUPO, MONICA T.; DE OLIVEIRA, ANDERSON R. M.. ASYMMETRIC SULFOXIDATION OF ALBENDAZOLE TO RICOBENDAZOLE BY FUNGI: EFFECT OF pH. Química Nova, v. 38, n. 7, p. 944-947, AUG 2015. (13/17658-9)

RIBEIRO BARROS, LUIZA SALDANHA; CARRAO, DANIEL BLASCKE; COSTA QUEIROZ, REGINA HELENA; MORAES DE OLIVEIRA, ANDERSON RODRIGO; DE GAITANI, CRISTIANE MASETTO. A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis. ELECTROPHORESIS, v. 37, n. 20, p. 2678-2684, OCT 2016. (13/17658-9, 10/17632-1)

MURIE, VALTER E.; MARQUES, LUCAS M. M.; SOUZA, GLORIA E. P.; OLIVEIRA, ANDERSON R. M.; LOPES, NORBERTO P.; CLOSOSKI, GIULIANO C.. Acetaminophen Prodrug: Microwave-Assisted Synthesis and in vitro Metabolism Evaluation by Mass Spectrometry. Journal of the Brazilian Chemical Society, v. 27, n. 6, p. 1121-1128, JUN 2016. (13/17658-9, 15/12811-9, 14/50265-3)

BARTH, THIAGO; HABENSCHUS, MAISA DANIELA; MOREIRA, FERNANDA LIMA; FERREIRA, LEANDRO DE SANTIS; LOPES, NORBERTO PEPORINE; MORAES DE OLIVEIRA, ANDERSON RODRIGO. In vitro metabolism of the lignan (-)-grandisin, an anticancer drug candidate, by human liver microsomes. DRUG TESTING AND ANALYSIS, v. 7, n. 9, p. 780-786, SEP 2015. (13/17658-9, 09/51812-0)

CARRAO, DANIEL BLASCKE; PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; MAURIZ MARQUES, LUCAS MACIEL; MILLER CROTTI, ANTONIO EDUARDO; PILON, ALAN CESAR; BOLZANI, VANDERLAN DA SILVA; BERRETTA, ANDRESA APARECIDA; MORAES DE OLIVEIRA, ANDERSON RODRIGO. In Vitro Metabolism of Artepillin C by Rat and Human Liver Microsomes. Planta Medica, v. 83, n. 8, p. 737-745, MAY 2017. (13/17658-9, 16/07597-0, 13/20094-0)

BOCATO, MARIANA ZUCCHERATO; BORTOLETO, MARCELA ARMELIM; PUPO, MONICA TALLARICO; MORAES DE OLIVEIRA, ANDERSON RODRIGO. A new enantioselective CE method for determination of oxcarbazepine and licarbazepine after fungal biotransformation. ELECTROPHORESIS, v. 35, n. 19, p. 8-pg., 2014-10-01. (13/17658-9)

FERNANDES, EDUARDO FELIPE ALVES; DE OLIVEIRA, ANDERSON R. M.; BARROS, VALERIA PRISCILA; GUARATINI, THAIS; LOPES, NORBERTO PEPORINE. Biomimetic Metabolism of Kaurenoic Acid Validated by Microsomal Reactions. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 30, n. 4, AUG 2020. (14/50265-3, 13/17658-9)

PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; DE GAITANI, CRISTIANE MASETTO; MORAES DE OLIVEIRA, ANDERSON RODRIGO. A new and fast DLLME-CE method for the enantioselective analysis of zopiclone and its active metabolite after fungal biotransformation. Journal of Pharmaceutical and Biomedical Analysis, v. 109, p. 192-201, MAY 10 2015. (12/21578-8, 13/17658-9)

BORTOLETO, MARCELA A.; BOCATO, MARIANA Z.; PUPO, MONICA T.; GAITANI, CRISTIANE M.; OLIVEIRA, ANDERSON R. M.. Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main Metabolites after Biotransformation by Fungi. Journal of the Brazilian Chemical Society, v. 26, n. 9, p. 1956-1966, SEP 2015. (13/17658-9, 11/17508-1)

MARQUES, LUCAS M. M.; CALLEJON, DANIEL R.; PINTO, LARISSA G.; DE CAMPOS, MICHEL L.; DE OLIVEIRA, ANDERSON R. M.; VESSECCHI, RICARDO; ADHIKARI, ACHYUT; SHRESTHA, RAM L. S.; PECCININI, ROSANGELA G.; LOPES, NORBERTO P.. Pharmacokinetic properties, in vitro metabolism and plasma protein binding of govaniadine an alkaloid isolated from Corydalis govaniana Wall. Journal of Pharmaceutical and Biomedical Analysis, v. 131, p. 464-472, NOV 30 2016. (13/17658-9, 13/16496-5, 14/13192-8, 14/23604-1)

MOREIRA, FERNANDA DE LIMA; HABENSCHUS, MAISA D.; BARTH, THIAGO; MARQUES, LUCAS M. M.; PILON, ALAN CESAR; BOLZANI, VANDERLAN DA SILVA; VESSECCHI, RICARDO; LOPES, NORBERTO P.; DE OLIVEIRA, ANDERSON R. M.. Metabolic profile and safety of piperlongumine. SCIENTIFIC REPORTS, v. 6, SEP 29 2016. (13/17658-9, 14/50265-3, 14/23604-1)

HABENSCHUS, MAISA DANIELA; MOREIRA, FERNANDA DE LIMA; LOPES, NORBERTO PEPORINE; DE OLIVEIRA, ANDERSON R. M.. In Vitro Inhibition of Human CYP450s 1A2, 2C9, 3A4/5, 2D6 and 2E1 by Grandisin. Planta Medica, v. 83, n. 8, p. 727-736, MAY 2017. (13/17658-9, 16/07597-0, 14/50265-3)

ROCHA, BRUNO ALVES; MORAES DE OLIVEIRA, ANDERSON RODRIGO; PAZIN, MURILO; DORTA, DANIEL JUNQUEIRA; RODRIGUES, ANDRESA PIACEZZI NASCIMENTO; BERRETTA, ANDRESA APARECIDA; PETI, ANA PAULA FERRANTI; BERALDO DE MORAES, LUIZ ALBERTO; LOPES, NORBERTO PEPORINE; POSPISIL, STANISLAV; et al. Jacobsen Catalyst as a Cytochrome P450 Biomimetic Model for the Metabolism of Monensin A. BIOMED RESEARCH INTERNATIONAL, v. 2014, p. 8-pg., 2014-01-01. (11/05800-0, 13/17658-9)

MOREIRA, FERNANDA DE LIMA; MOREIRA, MARCELA DE LIMA; PILON, ALAN CESAR; LOPES, NORBERTO PEPORINE. Leishmanicidal Effects of Piperlongumine (Piplartine) and Its Putative Metabolites. Planta Medica, v. 84, n. 15, p. 1141-1148, OCT 2018. (14/50265-3, 13/04188-4, 13/17658-9, 16/13292-8)