Abstract
The medicinal plants are the source for thousands of years. Species from Genus Piper has been highlighted because of its diverse pharmacological activities presented. Its prevalence is in tropical and subtropical regions of the globe. The Family Piperaceae has shown great diversity of secondary metabolites with biological activity, such as lignans, neolignans, terpenes, propenyl phenoles, chalcones, flavones, benzopyrans, lactones and amides alkaloids, which are characteristic metabolites. Among them, piplartine has significant cytotoxic activity in tumor cells, antifungal properties, anti platelet aggregation, anxiolytic, antidepressant and antiparasitic. The xenobiotics oxidative metabolism has been extensively researched in the last two decades by means of biological models, using perfused organs, isolated cells or cell fragments (microsomes). Its importance lies on the fact that new drug candidates, must pass through studies, to affect its safety and efficacy, making efforts to be taken in order to describe the involved metabolic pathway and also the characterization of enzymatic kinetics. The present work aims to develop and validate an analytical method for subsequent application in a study of piplartine in vitro metabolism using rat liver microsomes as well as characterization of enzymatic kinetics and the possible metabolites formed. The objectives of this project are as part of the FAPESP 2009/51812-0 project.
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