Penetration into dentinal tubules of a new endodontic irrigant

• Grant number: 24/10768-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2024
• End date: April 30, 2025
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Gisele Faria
• Grantee: Elda Olivia Nobre de Souza
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Sodium hypochlorite (NaOCl) solution is the most widely used irrigant in Endodontics due to itsantimicrobial properties and ability to dissolve organic tissue. However, it has limited action oninorganic tissue. In response to this limitation, the chelating agent ethylenediaminetetraaceticacid (EDTA) is employed as an irrigant following chemomechanical preparation of the root canalto remove the smear layer and inorganic debris. Additionally, continuous chelation involving aweak chelator like etidronic acid (HEDP), combined with NaOCl, has been studied to achieve continuous removal of the smear layer during chemomechanical preparation. Continuous chelation may offer improved antimicrobial action, dentin debris removal, and effective dissolution of pulpal tissue compared to NaOCl/EDTA. New substances are being investigated as alternatives to NaOCl/EDTA to enhance effective cleaning and disinfection of the root canal system and contribute to the success of endodontic treatment. In this context, Triton® (Brasseler), a new multifunctional 2-in-1 irrigating solution, has recently been developed with dual action: antimicrobial and smear layer/inorganic debris removal, as documented in the literature. The objective of the study will be to evaluate Triton®'s penetration into dentinal tubules compared to NaOCl solution and the combination of NaOCl + etidronic acid (EndoRinse HEDP, KDent) (NaOCl + HEDP). To achieve this, the crystal violet dye penetration method will be employed. After chemomechanical preparation of human premolar root canalsand staining of dentin with crystal violet dye, specimens will be randomly allocated into 3groups (n = 12 per group): Triton®, 4% NaOCl, HEDP + 4% NaOCl, and distilled water as an additional control (n = 3). Root canals will be irrigated with the test solutions, and roots will betransversely sectioned along their longitudinal axis at 3, 7, and 12 mm from the apex to obtaincervical, middle, and apical segments. The depth of irrigant penetration will be analyzed using astereomicroscope by measuring the depth of crystal violet dye decolorization in each root segment. Statistical tests will be chosen according to the distribution and homoscedasticity of the data and a significance level of 5% will be used.