Development, characterization and evaluation of the bioactivity of silver nanoparticles obtained by green synthesis using Brosimum gaudichaudii extract as a bioreductant.

• Grant number: 24/17398-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2025
• End date: December 31, 2025
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Regildo Márcio Gonçalves da Silva
• Grantee: Itallo Augusto Bevilacqua
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The synthesis of metal nanoparticles (NPMs) by biological systems is known as green synthesis or biosynthesis and can be carried out using prokaryotic and eukaryotic organisms or parts of them. In this synthesis strategy, instead of using toxic agents to reduce the metal ion, NPMs are obtained by biological components capable of reducing the metal. In this context, this project aims to develop the green synthesis and characterization of silver nanoparticles containing Brosimum gaudichaudii leaf extract in terms of extract concentration, AgNO3, pH and temperature/time. The aim is also to evaluate the antioxidant activity by means of DPPH radical scavenging tests; iron ion chelating by FRAP; lipid peroxidation inhibition (TBARS); ABTS-+ radical scavenging assay; nitric oxide (NO) scavenging activity and oxygen radical absorption capacity (ORAC-FL), and anti-glycating activity by the methods of relative mobility in electrophoresis (MRE), determination of free amino groups and inhibition of AGE formation of the silver nanoparticles containing extracts and comparing with the crude extracts of the leaves of B. gaudichaudii non-nude. gaudichaudii leaf extracts. It is hoped to prove that the green synthesis process for obtaining silver nanoparticles containing B. gaudichaudii leaf extract, as well as elucidating their antioxidant and anti-glycating activity, with the possibility of applying AgNPs in future formulations such as food supplements, functional foods and pharmacologically active products in the prevention and/or damage caused by oxidative stress and protein glycation, thus allowing an increase in scientific information for the safe use of this species.