In vitro study of the interaction between phytocannabinoids and xenogeneic biomaterial in the differentiation of osteoblastic cells

• Grant number: 25/07842-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2025
• End date: May 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Michelle Bianchi de Moraes
• Grantee: Vitória Karina Lima da Trindade
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Diseases that affect bone metabolism can manifest themselves both in the body and in the oral cavity, since bone defects can originate from various causes, such as tooth loss, periodontal disease, trauma, tumors and degenerative pathologies. However, the larger the bone defect, the more challenging its regeneration becomes, even considering the natural regenerative properties of bone tissue. In many cases, the association of grafts or biomaterials becomes necessary to achieve better results. At the same time, there is a growing interest in the use of cannabis for therapeutic purposes, especially through the phytocannabinoids present in plants and their interaction with the endocannabinoid system, naturally present in the human body. This interaction reveals a complex network of chemical signals and cellular receptors synthesized in various tissues, including bone tissue. Therefore, the present study aims to evaluate the phytocannabinoids cannabidiol (CBD), ¿9-tetrahydrocannabinol (THC) and cannabigerol (CBG), produced in Brazilian climate and soil, in different concentrations, in the MC3T3-E1 osteoblast cell line, in association with the xenogeneic biomaterial Hemospon®. The research will be conducted through an in vitro study, in which cellular activity and differentiation will be analyzed. Cellular assays will include viability tests, evaluation of morphology, cell migration and proliferation, quantification of total protein content, in addition to alkaline phosphatase activity and formation of mineralization nodules. The data obtained will be subjected to statistical analysis, with initial application of the normality test to define the most appropriate statistical test. The significance level adopted will be 5% (p < 0.05). (AU)