Analysis of the Physicochemical and Biological Properties of an Experimental Cement and a New Light-Curable Hydrogel Material (ODNEFILL and ODNECURE) for Root Canal Filling.

• Grant number: 25/03368-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2025
• End date: June 30, 2028
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marco Antonio Hungaro Duarte
• Grantee: Abel Victor Isidro Teves Cordova
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The filling should ensure proper filling and airtight sealing of the root canals, and the filling cement should, whenever possible, penetrate the dentin tubules. This underscores the importance of cement, which is essential for forming an effective barrier between the channel walls and the cone. The properties of endodontic cements are widely studied with the aim of improving their characteristics at the biological, chemical and mechanical levels. Over time, many types of cements have been developed, from those based on zinc oxide and eugenol, through softwoods to hydraulic cements. However, new formulations continue to be tested, since, despite the advantages of the cements already mentioned, issues such as solubility, dimensional stability and sealing are still weak or little studied points. The present project aims to determine the physicochemical and biological properties of an experimental cement based on Polyurethane and a new light-curing hydrogel material (OdneFill and OdneCure) for root canal filling. These will be compared to the performance of commercial cements used in clinical practice, such as AH Plus Jet , Bioroot RCS and BC Sealer. The properties studied will include: Setting time, which will be evaluated according to ASTM C 266 - 2008 for hydraulic cements; Runoff; Radiopacity, evaluated according to ISO 6876/2001 standards; Volumetric variation, which will be analyzed by means of micro-computed tomography after 7 days of filling; pH, which will be tested in periods of 03, 24, 72 and 168 hours; Solubility, following specific standards. In addition, biological tests, such as the cell viability assay (MTT Assay) and subcutaneous implantation in rats, will be carried out to assess their biocompatibility and tissue response. The sealing ability and aging of materials through techniques such as Differential Scanning Calorimetry (DSC), energy dispersive X-ray spectroscopy (EDS), Raman spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy will also be investigated, both before and after aging. The data obtained in the tests will be statistically compared (significance level of 5%) with the results of the endodontic cements AH Plus Jet, BC Sealer and BioRoot RCS (AU)