Development and characterization of solid lipid nanoparticles based on Dipteryx alata oil for evaluation of antioxidant, antiglycation and anti-aging activities

• Grant number: 25/18421-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2025
• End date: October 31, 2027
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Regildo Márcio Gonçalves da Silva
• Grantee: Monique Maria de Oliveira Costa
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The Brazilian Cerrado is home to native species with high biotechnological potential, such as Dipteryx alata Vogel (baru), whose nuts provide oil rich in unsaturated fatty acids, polyphenols, carotenoids, and tocopherols. Despite its recognized antioxidant, anti-inflammatory, and functional properties, the oil is highly susceptible to oxidation, limiting its application. Nanotechnology, particularly solid lipid nanoparticles (SLNs), emerges as a promising strategy for preserving and enhancing the oil's bioactive constituents, increasing its stability, bioavailability, and efficacy. Therefore, this project aims to develop a method for producing SLNs containing D. alata oil and evaluate their antioxidant, antiglycation, and antiaging activities. The steps include: sustainable extraction of baru oil by partner company Atina - Ativos Naturais; preparation of SLNs by fusion of solid lipids and emulsification with biocompatible surfactants, followed by drying; Physicochemical characterization (size, zeta potential, morphology by SEM, and FTIR spectroscopy) and determination of encapsulation efficiency; stability testing; and controlled-release assays. Biological activities will be evaluated using various in vitro methodologies: DPPH, FRAP, ABTS, and ORAC assays for antioxidant activity; determination of inhibition of bovine serum albumin glycation and AGE formation for antiglycation activity; and enzymatic inhibition of collagenase, elastase, and tyrosinase for antiaging activity. Encapsulation of baru oil in NLS is expected to increase protection against oxidative degradation, improve the bioavailability of bioactive compounds, and amplify functional effects compared to the natural oil. The results may support the development of innovative products in the food, cosmetic, and pharmaceutical sectors, in addition to contributing to the socioeconomic valorization of baru and the sustainable use of Cerrado biodiversity. (AU)