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Evaluation of the feed suplementation with mannanoligosaccharide to Nile tilapia in the immunological response, productive performance and challenge with Streptococcus agalactiae

Grant number: 10/00319-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2010
Effective date (End): December 31, 2010
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Fabiana Pilarski
Grantee:Nycolas Levy Pereira
Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

This work will study the effects of the addition of mannanoligosaccharide in the Nile tilapia diet in four levels, during 45 days, in the productive performance, number of leukocytes and total thrombocytes, differential leukocyte counts, hematocrit tax, total protein, lisozyma, burst of the leukocytes, intestine morphology and survival after the challenge with the Streptococcus agalactiae bacteria. Will be used 450 juveniles of Nile tilapia weighting about 30 and 50 g distributed aleatory in 30 tanks with the capacity of 310 liters (5 treatments, 3 repetition, 15 fishes/tank), that will be supplied with water from an artesian well with continuous aeration. During the experimental period the fishes will be feed with five experimental rations, with 0.4, 0.8, 1.0 and 1.5%, and one ration without the mannanoligosaccharide (0). The diets will be served twice a day until the fishes are feedstuff. After the feed period (time 0, 30 and 45 days after the feeding), the fishes will be captured and the blood will be withdrawn for the evaluation of physiological parameters (glucose, total protein, count of blood cells, hematocrit and hemoglobin), immunological (lisozyma and burst of leukocytes), histological (intestine morphology) and the productive performance (growth and weight gain). After 45 days the fishes will be submitted to a challenge with Streptococcus agalactiae. The fishes will continue to be feed daily with the experimental diets for two weeks more taking note daily the clinical signs of bacterial and mortalities taxes.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEVY-PEREIRA, NYCOLAS; MORO DE SOUSA, RICARDO LUIZ; SAKABE, ROBERSON; SEBASTIAO, FERNANDA DE ALEXANDRE; URBINATI, ELISABETH CRISCUOLO; PILARSKI, FABIANA. DIETARY MANNAN-OLIGOSACCHARIDE INCREASES REACTIVE OXIGEN SPECIES PRODUCTION BUT DECREASES SERUM LYSOZYME IN HIGH LEVELS OF INCLUSION FOR NILE TILAPIA. BOLETIM DO INSTITUTO DE PESCA, v. 46, n. 3 2020. Web of Science Citations: 0.
LEVY-PEREIRA, NYCOLAS; YASUI, GEORGE SHIGUEKI; CARDOZO, MARITA VEDOVELLI; DIAS NETO, JOSE; VAZ FARIAS, THAIS HELOISA; SAKABE, ROBERSON; DE PADUA, SANTIAGO BENITES; PILARSKI, FABIANA. Immunostimulation and increase of intestinal lactic acid bacteria with dietary mannan-oligosaccharide in Nile tilapia juveniles. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, v. 47, 2018. Web of Science Citations: 0.
NYCOLAS LEVY-PEREIRA; GEORGE SHIGUEKI YASUI; MARITA VEDOVELLI CARDOZO; JOSÉ DIAS NETO; THAÍS HELOÍSA VAZ FARIAS; ROBERSON SAKABE; SANTIAGO BENITES DE PÁDUA; FABIANA PILARSKI. Immunostimulation and increase of intestinal lactic acid bacteria with dietary mannan-oligosaccharide in Nile tilapia juveniles. REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, v. 47, p. -, 2018.

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