The effect of low-fluoride dentifrices supplemented with calcium on the demineralization and biofilm in situ

• Grant number: 07/05915-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2008
• End date: December 31, 2008
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Alberto Carlos Botazzo Delbem
• Grantee: Jackeline Gallo Do Amaral
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The experiment predicts a double-blind crossover in situ study, in which 10 voluntaries will participate in four experimental phases of 7 days, with intervals of 7 days between them, wearing a dispositive on the palate for each experimental phase containing 4 blocks of bovine enamel (obtained through section and polishing from bovine incisor teeth, previously selected through surface microhardness (initial SMH)). Cariogenic challenges will be produced with 30% sucrose solution, 6-times per day. The groups are defined as: (PC) positive control: dentifrice with 1100 µg F/g (Crest®); (NC) negative control: non-fluoride dentifrice (Placebo); (D1) dentifrice with 500 µg F/g supplemented with Ca; (D2) dentifrice with 500 µg F/g. After the experimental period, the superficial microhardness (SMH1) will be analyzed, in order to calculate the percentage of variation of the superficial microhardness (%SMHC), and values will be transformed in percentage of mineral volume (% vol min). The fluoride, calcium and phosphate determination will be performed by pre-established tests. Data will be submitted to normality and homogeneity tests to proper perform statistical analysis. Based on this study, it is expected to verify the effect of low concentration fluoride dentifrices supplemented with calcium on enamel demineralization process and the biofilm formed in situ. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO AMARAL, JACKELINE GALLO; SASSAKI, KIKUE TAKEBAYASHI; RODRIGUES MARTINHON, CLEIDE CRISTINA; BOTAZZO DELBEM, ALBERTO CARLOS. Effect of low-fluoride dentifrices supplemented with calcium glycerophosphate on enamel demineralization in situ. AMERICAN JOURNAL OF DENTISTRY, v. 26, n. 2, p. 75-80, APR 2013. (07/05915-6)