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Evaluation of lignin biodegradation by extracts of White-rot fungi

Grant number: 08/06039-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2008
Effective date (End): September 30, 2009
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Principal researcher:Adriane Maria Ferreira Milagres
Grantee:Marcio Andre de Lima Silveira
Home Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

This work's purpose is to evaluate a possible synergistic action in the lignin biodegradation of two different woods, Eucalyptus grandis (hard wood) and Pinus radiata (soft wood), through low molecular weight compounds and enzymes produced by three different white-rot fungi, Trametes versicolor, Lentinula edodes and Perenniporia medulla-panis. At first, the lignin will be extracted from wood by the dioxane method slightly adapted. The next phase of this study will be concerned on the growth of cultures of the three fungi in liquid media for the medium extracts achievement. The extracts with the maximum of enzymatic activity and iron reducing capability will be tasted in the biodegradation of lignin as such on the discoloration of three different dyes, RBBR, Poly R-4778 and Congo red.