Use of fluorescence images, laser-induced breakdown spectroscopy and chemometrics strategies to Greening disease study in citrus plantation

• Grant number: 07/08618-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2008
• End date: May 31, 2010
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Débora Marcondes Bastos Pereira
• Grantee: Fabiola Manhas Verbi Pereira
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated research grant: 04/15965-2 - Implementation and development of Laser Induced Breakdown Spectroscopy (LIBS), AP.TEM

Abstract

Brazil is the biggest producer of orange (Citrus sinensis) and the majority of this fruit is intended to industries of concentrated orange juice that was responsible for revenue more than US$ 2 billion in export trade (2007). São Paulo State contributes with 70% of national production of orange and generates 98% of manufactured orange juice. Notwithstanding, the performance of this sector have been hardly exposed to danger due to diseases and pests, as example the Greening that was detected on Citrus Brazilian plantation in 2004. Since the first reports up to this moment, this disease has no method for early detection. The Greening is increasing in an exponential way and affects approximately 4% of orchard in a central region of São Paulo State. Nowadays, the alternatives for producers are inspection of orchards, elimination of infected trees and air pulverization for control of insects. The accumulated damages are high, but are not higher than what can happen with the country economy. In the case of the disease will be not ruled as soon as possible. With this panorama, the scope of this research plane is the development of a method for early diagnoses of Greening. For this purpose, the fluorescence images will be used, specially their generated by LIFI (Laser-Induced Fluorescence Imaging) and, analyses of variation in a mineral composition of leaves due to the disease using LIBS (Laser-Induced Breakdown Spectroscopy). The obtained data will be evaluated with chemometrics tools, such as, PCA (Principal Component Analysis). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERBI PEREIRA, FABIOLA MANHAS; BASTOS PEREIRA MILORI, DEBORA MARCONDES; PEREIRA-FILHO, EDENIR RODRIGUES; VENANCIO, ANDRE LEONARDO; TAVARES RUSSO, MARIANA DE SA; DO BRASIL CARDINALI, MARCELO CAMPONEZ; MARTINS, POLYANA KELLY; FREITAS-ASTUA, JULIANA. Laser-induced fluorescence imaging method to monitor citrus greening disease. COMPUTERS AND ELECTRONICS IN AGRICULTURE, v. 79, n. 1, p. 90-93, OCT 2011. (07/08618-2)

VERBI PEREIRA, FABIOLA MANHAS; BASTOS PEREIRA MILORI, DEBORA MARCONDES; VENANCIO, ANDRE LEONARDO; TAVARES RUSSO, MARIANA DE SA; MARTINS, POLYANA KELLY; FREITAS-ASTUA, JULIANA. Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools. Talanta, v. 83, n. 2, p. 351-356, DEC 15 2010. (07/08618-2)

VERBI PEREIRA, FABIOLA MANHAS; BASTOS PEREIRA MILORI, DEBORA MARCONDES. Investigation of the stages of citrus greening disease using micro synchrotron radiation X-ray fluorescence in association with chemometric tools. Journal of Analytical Atomic Spectrometry, v. 25, n. 3, p. 351-355, 2010. (07/08618-2)

VERBI PEREIRA, FABIOLA MANHAS; BASTOS PEREIRA MILORI, DEBORA MARCONDES; PEREIRA-FILHO, EDENIR RODRIGUES; VENANCIO, ANDRE LEONARDO; TAVARES RUSSO, MARIANA DE SA; MARTINS, POLYANA KELLY; FREITAS-ASTUA, JULIANA. Fluorescence images combined to statistic test for fingerprinting of citrus plants after bacterial infection. ANALYTICAL METHODS, v. 3, n. 3, p. 552-556, MAR 2011. (07/08618-2)