EFFECT OF RADIATION WITH LOW INTENSITY LASER IN THE PRE-OSTEOBLAST IN VITRO: CELL VIABILITY AND MMPS -2 AND -9 EXPRESSION ANALYSIS.

• Grant number: 08/11391-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2009
• Effective date (End): June 30, 2010
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Camila Peres Buzalaf
• Grantee: Flávia Amadeu de Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Low intensity lasers are electromagnetic waves constituted by photons at the same frequency with specific wave length that act in the cellular biomodulation. It works as a therapeutic agent rebalancing and normalizing the cellular activity. Also, it has analgesic, anti-inflammatory activities and accelerates injury repair. Osteoblasts are bone cells able to produce mineralized matrix. However, factor involved in the process of extracellular matrix remodelation remain unclear, especially in the bone remodelation, and among him to be a matrix metalloproteinases (MMPs). Regarding the effect of the laser in osteoblasts, studies have been shown positive results like increased alkaline phosphatase synthesis that indicates greater cellular activity. The aim of this work is to evaluate in vitro the influence of phototherapy with low intensity laser in pre-osteoblast viability and matrix metalloproteinases activity (MMPs). MC3T3 will be plated at the density of 5x10²(murine pre-osteoblast ATCC) in 96 well plates and will be irradiated either with red laser (660 nm) or infrared (780 nm) at the doses of 3 and 5 J/cm²and a control group (no radiation). Pre-osteoblast will be collected and evaluated regarding the cellular viability (cellular counts, MTT reduction and neutral red capitation) and the enzymatic activity (MMP2 and MMP9) will be evaluated by zymography. After protein isolation from pre-osteoblasts they will be submitted by zymography. The gels from control and treated groups will be scanned using ImageScanner and analyzed by Kodak Molecular Imaging Software®. Statistical analysis will be performed by using ANOVA, Kruskal-Wallis and t test according the indication (p< 0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, FLAVIA AMADEU; MATOS, ADRIANA ARRUDA; MATSUDA, SANDRA SATIKO; RABELO BUZALAF, MARILIA AFONSO; BAGNATO, VANDERLEY SALVADOR; DE ANDRADE MOREIRA MACHADO, MARIA APARECIDA; DAMANTE, CARLA ANDREOTTI; DE OLIVEIRA, RODRIGO CARDOSO; PERES-BUZALAF, CAMILA. Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 169, p. 35-40, APR 2017. (08/11261-1, 10/14289-4, 07/08367-0, 08/11391-2)

OLIVEIRA, FLAVIA A.; MATOS, ADRIANA A.; SANTESSO, MARIANA R.; TOKUHARA, CINTIA K.; LEITE, ALINE L.; BAGNATO, VANDERLEY S.; MACHADO, MARIA A. A. M.; PERES-BUZALAF, CAMILA; OLIVEIRA, RODRIGO C.. Low intensity lasers differently induce primary human osteoblast proliferation and differentiation. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 163, p. 14-21, OCT 2016. (08/11261-1, 07/08367-0, 08/11391-2)