Intracellular peptides in Parkinson\'s disease and in schizophrenia.

Author(s)

Cecilia Cerqueira Café Mendes

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-09-18

Examining board members

Luiz Roberto Giorgetti de Britto; Jair Ribeiro Chagas; Vanessa Rioli; Alexandre Keiji Tashima; Ivarne Luis dos Santos Tersariol

• Advisor: Luiz Roberto Giorgetti de Britto

Abstract

The thesis is divided in one chapter, which was dedicated to peptidomic analysis of the striatum in the 6-OHDA mice model and also to oligopeptidase activity such as EP 24.15 and EP 24.16. The second chapter refers to data obtained during a research internship in the Max-Planck Institute of Psychiatry, Munich, Germany, in which we ran peptidomic analysis of post-mortem samples of the anterior temporal lobe and corpus callosum from schizophrenia patients. Both projects used mass spectrometry techniques such as isotopic labeling and label free applied to LC-MS/MS. In the Parkinsons disease model we observed significant changes in 5 different peptide fragments, and for schizophrenia samples, 2 peptide fragments were biologically different. The resulting data acquired in both projects lead to a substantial increase in prospective possibilities to new targets associated to the disorders studied here. (AU)

• FAPESP's process: 09/53515-2 - Analysis of oligopeptidases and intracellular peptides and their physiopathological implications in neurodegenerative diseases
• Grantee: Cecília Cequeira Café Mendes
• Support Opportunities: Scholarships in Brazil - Doctorate