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| Author(s): |
Joana Mona e Pinto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-12-08 |
| Examining board members: |
Jose Roberto Machado Cunha da Silva;
Francisco Javier Hernandez Blazquez;
Kenio de Gouvêa Cabral;
Maria José Tavares Ranzani de Paiva;
Renata Guimarães Moreira Whitton
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| Advisor: | Jose Roberto Machado Cunha da Silva |
| Abstract | |
Teleosts subjected to stress show increase in glucocorticoids levels, this triggers a metabolic reorganization, resulting in the animal immunosuppression, this let animals susceptible to potential pathogens. The antibiotics are commonly used to control bacterial diseases, but their indiscriminate use can lead to selection of resistant pathogenic strains. A viable alternative is to work on prevention, through the use of immunostimulant dietary additives. Flavonoids and ascorbic acid (AA) are known for their anti-inflammatory, antioxidant and anti-stress activity. For this reason, the present study aimed to evaluate their influence in the growth performance; the gut; the innate immunity parameters of rainbow trouts in ideal conditions and after an exogenous glucocorticoid application (dexamethasone). For this, two experiments were performed: 1- groups control (GC) and the additive (GA) for ninety days. 2 - with four groups control (GC), additive (GA), dexamethasone (GD) and additive + dexamethasone (GAD) lasting thirty ninety days. In the first experiment, the additive increased epithelial height at the initial intestine, in addition to decreased mucus cell density of the pyloric caeca, and the increase in the initial intestine. In the second experiment, the additive decreased epithelial height in pyloric caeca and initial intestine. The exogenous glucocorticoid caused animal weight loss, and epithelial height decrease in all intestinal portions. Positive results was observed on GAD leukocytes number, so the additive was differential and seemed to compensate the glucocorticoids actions. The results indicate that the use of ascorbic acid and flavonoids has advantages in stress situations. (AU) | |
| FAPESP's process: | 10/19284-0 - The treatment effects of ascorbic acid and flavonoids in the gut and innate imune system of a rainbow trout (Oncorhynchus mykiss, Walbaum, 1972). |
| Grantee: | Joana Mona e Pinto |
| Support Opportunities: | Scholarships in Brazil - Doctorate |