Ultrastructural characterization of 5-brome-2-deoxyuridine (BrdU) immunoreactives cells in adult ventricular and subventricular zone and its relationship with regulating peptide CART.

Author(s)

Carlos Alexandre dos Santos Haemmerle

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-03-17

Examining board members

Ii Sei Watanabe; Dania Emi Hamassaki; Ruy Gastaldoni Jaeger; Paula de Carvalho Papa; Marimelia Aparecida Porcionatto

• Advisor: Ii Sei Watanabe

Abstract

The major neurogenic niche in adult brains surrounds the lateral ventricles, but the identity of the cell that initiates this process in controversial. There is an innervation made by the CART peptide that may lead to perspectives for understanding its role in modulation of neurogenesis. We propose to study the ultrastructural cytoarchitecture of proliferative cells in this region and its innervation by CART immunoreactive axons. We used adult rats and mice, prepared for ultrastructural and neurochemical analysis by transmission and high-resolution scanning electron, light and laser confocal microscopes. The proliferation and innervation studies occured with the S-phase marker BrdU and anti-Brdu, anti-CART, anti-DCX, anti-GFAP, anti-GFP antibodies. Each sort of cells in neurogenic niche presented a proper density of BrdU staining. We identified the cells lining the ventricle being innervated by axons. The major density of CART innervation occurs along the pathway of neurons in maturation process. (AU)

• FAPESP's process: 10/17519-0 - Ultrastructural characterization of 5-bromo-2-deoxyuridin (BrdU) immunoreactive cells in adult ventricular and subventricular zone and its relationship with the regulating peptide CART
• Grantee: Carlos Alexandre dos Santos Haemmerle
• Support Opportunities: Scholarships in Brazil - Doctorate