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| Author(s): |
Gisele Cristiane de Melo Dias
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-03-20 |
| Examining board members: |
Maria Ines Borella;
Sérgio Ricardo Batlouni;
Sergio Ferreira de Oliveira;
Elizabeth Romagosa;
Fábio Siviero
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| Advisor: | Maria Ines Borella |
| Abstract | |
Adult males of P. lineatus had their gonads used according routines of light microscopy and transmission electron microscopy. For cell culture, the testes were enzymatically digested; testicular suspension was separated by discontinuous gradient with Percoll followed by adhesion differential plating and the cells were cultured. The enrichment of the spermatogonia was carried by flow cytometry. The testes present three regions with similar distribution of cell types, and nuclear diameter of germ cells decreases significantly during spermatogenesis. The spermatogonia cultured for 15 days with medium for cell proliferation, resulted in large cell agglomerates which were characterized with the antibodies anti-Vasa, anti-GFRa1 and anti-anti-OCT4. The cultures that receiving medium for cell differentiation showed slow proliferation process of primary spermatogonia compared to cell culture medium suggestive for cell differentiation. (AU) | |
| FAPESP's process: | 10/01899-9 - CHARACTERIZATION, ISOLATION AND CULTURE OF PRIMARY SPERMATOGONIA OF Prochilodus lineatus (Valencienes, 1847). |
| Grantee: | Gisele Cristiane de Melo Dias |
| Support Opportunities: | Scholarships in Brazil - Doctorate |