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Human Papillomavirus infection in the oral fluid of HIV-infected men: Prevalence and relation with risk factors

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Author(s):
Karen Eliane de Oliveira Gaester
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT)
Defense date:
Examining board members:
Jorge Simão do Rosário Casseb; José Eduardo Levi; Roberto José Carvalho da Silva
Advisor: Jorge Simão do Rosário Casseb
Abstract

Human Papillomavirus is one of the most common sexually transmitted infection worlwide. The natural history of oral HPV infection is unclear and its risk factors have not been explored. Immunocompromised people, as exemplified by HIV patients, are at high risk for HPV-infection. Objectives: To determine the prevalence of HPV in the oral tract of HIV-1-positive male subjects and its association with risk factors. Case series: A total of 283 oral wash samples were analyzed. All samples were processed by washing processes, DNA extraction, DNA amplification by conventional PCR (MY 09/11), agarose gel electrophoresis and HPV DNA positive samples were submitted to HPV genotyping by hybridization. Results: HPV genotyping revealed of types 06, 16, 44, 51, 56, 58, 62, 66, 67, 72, 83 and 84; major high risk HPV type identified was HPV-66 and low risk types were HPV-6 and 83. Regarding risk factors, smoking was the only risk factor considered significant [OR (CI) = 10,04 (1,98 - 50,92), p>0,01] for the oral HPVinfection in HIV-1-infected subjects in São Paulo, Brazil. Discussion: The prevalence of oral HPV is highly variable due to factors such as method of collection and analysis. Although data on HPV infection in men are scarce, studies show that most Brazilian men are infected by the virus. The main risk factors are unprotected sexual intercourse, but other factors for this infection have been described elsewhere including smoking, alcohol consumption and HIV-positive serostatus. Conclusion: the prevalence of oral HPV in the individuals studied was 3.5% and among the types analyzed, most are not found in HPV vaccine commercially available. (AU)

FAPESP's process: 11/16094-9 - Prevalence of HPV in oral fluid and its risk factors in HIV-1-infected men.
Grantee:Karen Eliane de Oliveira Gaester
Support Opportunities: Scholarships in Brazil - Master