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| Author(s): |
Camila Araújo Bernardino Garcia
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2015-01-30 |
| Examining board members: |
Valeria Paula Sassoli Fazan
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| Advisor: | Hélio Rubens Machado |
| Abstract | |
Background: Hydrocephalus is a syndrome resulting from the current unbalance between the formation and absorption of cerebrospinal fluid (CSF), and consequent accumulation within the cerebral ventricles. Clinically, hydrocephalic children can present several neurological disorders, not always reversed with treatment, even after the development of more and more sophisticated shunt systems (1). Although lesions of hydrocephalus be multifactorial, it is known that oxidative stress is one of the mechanisms involved. The Edaravone is a new antioxidant drug with neuroprotective effects but has not been tested in hydrocephalus (2). Objectives: To evaluate the neuroprotective response of Edaravone on experimental hydrocephalus in young rats. Methodology: Hydrocephalus was induced in pup rats by injecting kaolin into the cisterna magna. The animals were divided into three groups with 10 rats each one: control (C); untreated hydrocephalus (HNT); hydrocephalus treated with 20mg/kg Edaravone (HTE). The treated group receive the drug every day from the first day after induction. The animals were weighed daily, assessed by behavioral tests and magnetic resonance examination. All animals were sacrificed 14 days after hydrocephalus induction and the brains processed for histological, immunohistochemical and biochemical analysis. In the analysis of caspase-3 treated animals showed Edaravone a smaller number of cells in the process of apoptosis. Results: From the third to the eighth day after the hydrocephalus induction, the animals gained less weight than controls. However, from the ninth day of induction, weight gain was similar in the 3 experimental groups. In the open field test the performance of hydrocephalic rats receiving Edaravone was better than those who did not receive the drug. In immunohistochemistry for GFAP in the corpus callosum, the control rats did not exhibit reactive astrocytes. On the other hand, the animals of HNT group showed intensely labeled astrocytes with thick extensions, while the animals in the HTE group the astrocytes were more delicate and with thin extensions. Conclusion: The use of Edaravone showed a tendency to decrease in cell apoptosis improved the behavioral response and slightly reduced astrocyte activity evidenced by GFAP immunostaining in the corpus callosum of young rats with hydrocephalus (AU) | |
| FAPESP's process: | 13/04130-6 - Neuroprotective effects of Edaravone in experimental hydrocephalus induced in Wistar rats |
| Grantee: | Camila Araujo Bernardino Garcia |
| Support Opportunities: | Scholarships in Brazil - Master |