Roles of collagen I glycation and high glucose concentration on fibroblast migration.

Author(s)

Maíra Estanislau Soares de Almeida

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2015-10-22

Examining board members

Marinilce Fagundes dos Santos; Hernandes Faustino de Carvalho; Patricia Gama; Ruy Gastaldoni Jaeger; Marisa Passarelli

• Advisor: Marinilce Fagundes dos Santos

Abstract

We evaluated the effects of collagen glycation (GC) and high glucose concentrations on fibroblasts migration. Fibroblasts derived from control and diabetic rats (D) and NIH-3T3 cells were cultured under 5 mM or 30 mM glucose (HG). For glycation, glyoxylic acid was used. The GC showed lower tensile strength and elasticity. Fibroblasts migrated less in HG and over the GC. D cells did not move on GC, showed less β141 integrin and a higher expression of smooth muscle α-actin. The viscoelasticity of the cytoskeleton was lower in D cells, especially on the GC. On fibronectin, NIH-3T3 cells under HG had fewer stress fibers and showed impaired contraction at the rear, presenting long tails. The expression of myosin IIA (MIIA), IIB (MIIB) and MRLC has not changed, but the phosphorylation of MII decreased. The distribution of MIIB became more diffuse, while MIIA has not changed. Cells under HG exerted less force on the substrate. The migration of fibroblasts in hyperglycemic environment is impaired, especially on GC, partly due to a reduction of cell contractility. (AU)

• FAPESP's process: 12/15963-6 - ROLE OF OXIDATIVE STRESS AND EXTRACELLULAR MATRIX GLYCATION ON MIGRATION OF DIABETIC SKIN FIBROBLASTS
• Grantee: Maíra Estanislau Soares de Almeida
• Support Opportunities: Scholarships in Brazil - Doctorate