Development and validation of analytical methos for the determination of streptomycin and ddihydrostreptomycin residues in milk (ELISA, LC-APCI-MS/MS QToF)

Author(s)

Renata Cabrera de Oliveira

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
• Defense date: 2009-07-31

Examining board members

Felix Guillermo Reyes Reyes; Miguel Machinski Junior; Jose Augusto Rizzato Paschoal

• Advisor: Felix Guillermo Reyes Reyes

Abstract

Veterinary drugs pose a risk to human health when there residual levels in foods exceed the maximum residue limit (MRL). Knowledge of the extent of human exposure to these compounds is necessary to provide consumer protection. To achieve this, it is essential that validated analytical methods are available to ensure reliable results. The aim of the present work was to develop and validate analytical methods for the analysis of streptomycin and dihydrostreptomycin residues in milk, and to employ these methods for analysis of samples marketed in the region of Campinas, São Paulo. The developed and/or validated methods include enzyme immunoassay (ELISA) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS QToF). Method validation was performed according to the recommendations established by European Community and IUPAC. The following parameters were evaluated: analytical curve, linearity, sensitivity, precision (intra and inter-day repeatability), accuracy and the detection and quantification limits. The results demonstrated the effectiveness of ELISA and LCMS/MS methods for trial and confirmatory analyses, respectively, of streptomycin and dihydrostreptomycin in milk at concentrations below the established MRL (200µg kg-1). In the analyzed samples streptomycin and dihydrostreptomycin were not found at detectable levels (AU)

• FAPESP's process: 07/02233-1 - Development and validation of analytical methods for the determination of streptomycin and dihydrostreptomycin residues in milk (ELISA, LC-APCI-MS/MS QToF)
• Grantee: Renata Cabrera de Oliveira
• Support Opportunities: Scholarships in Brazil - Master