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| Author(s): |
Jéssica Santana Bernachi
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2014-11-18 |
| Examining board members: |
Maria Marluce dos Santos Vilela;
Maria Notomi Sato;
Ronei Luciano Mamoni
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| Advisor: | Taís Nitsch Mazzola; Maria Marluce dos Santos Vilela |
| Abstract | |
Therapeutic vaccination with monocyte-derived dendritic cells (MDDC) has been associated with viral load control in immunized HIV-infected adults, but its effects in children were not explored yet. We aimed to evaluate the function of MDDC isolated from vertically HIV-infected children and adolescents under combined antiretroviral therapy. Patients were recruited at the Out-Patients Unit at the University of Campinas, which 25 patients had undetectable viral load and 14 patients had viral load ?1000 RNA copies/mL. In addition, 20 healthy children and adolescents served as controls. Monocytes obtained from peripheral blood were cultured for six days with GM-CSF and IL-4 to get the MDDC, which were assessed by flow cytometry for CD40, CD80, CD83, CD86, DC-SIGN and HLA-DR expression. T cell proliferation and cytokine expression and production were evaluated in cocultures of MDDC pulsed with aldrithiol-2-inactivated HIV-1 and autologous lymphocytes. Hemogram, myeloid and plasmacytoid dendritic cells and CD3, CD4 and CD8 lymphocytes were evaluated in the peripheral blood. MDDC from HIV-infected children exhibited a similar phenotype to those in healthy individuals and were able to induce a Th1-type cytokine production; however, T lymphoproliferation was impaired in children with viral load ?1,000 RNA copies/mL. MDDC generated in vitro boosted HIV-specific cellular immune responses in vertically HIV-infected children and could be potential candidates for MDDC therapeutic immunization (AU) | |
| FAPESP's process: | 12/06525-5 - Cytokine gene expression from cocultures of lymphocytes and dendritic cells derived from monocytes of HIV-infected children and adolescents |
| Grantee: | Jéssica Santana Bernachi |
| Support Opportunities: | Scholarships in Brazil - Master |