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| Author(s): |
Guilherme Rodrigues Teodoro
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São José dos Campos. 2016-03-21. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos |
| Defense date: | 2016-02-04 |
| Advisor: | Cristiane Yumi Koga Ito; Marcos José Salvador |
| Abstract | |
The aim of this study was to evaluate the effectiveness against Candida albicans of fraction enriched in phenolic (FE) of Buchenavia leaves tomentosa beyond substances gallic acid (GA), corilagin, kaempferol and vitexin, phenolic substances previously detected in the aqueous extract of B. tomentosa, free or encapsulated in cyclodextrins. Microdilution test with standard strains and clinical isolates besides the chemical analysis of FE by mass spectrometry (ESI-MS) were carried out. The acetone extract was the FE and AG was the most efficient phenolic substance against C. albicans. The effect of FE and AG against the virulence factors of C. albicans was also analyzed. FE and AG were encapsulated into 2-hydroxypropyl-beta-cyclodextrin (HP-β-CD) and had their chemical analyses made. MICs and MFCs of encapsulated have been determined. Solely the GA encapsulated had its anti-biofilm and in vivo action verified. Cytotoxicity of free and encapsulated GA and FE were determined. The MIC ranged from 5,0 to 0,625 mg/ml for GA and 2,5 and 0,019 mg/ml for FE. MFCs values were not found. All phenolics molecules were found in FE by ESI/MS. AG and FE had a direct effect on virulence factors of C. albicans, except on the secretion of exoenzymes. There was no difference in the CIM between free and encapsulated substances. The anti-biofilm effect was better in GA encapsuladed than its free form. A clinical improvement of sugestives erythematous lesions on the palate of rats was observed, although the hyphaes were not found in the palatar mucosa. The cytotoxicity for all substances was moderated. After the analysis, we observed the anti-C. albicans effect of GA and FE. AG encapsulated showed promising anti-biofilm effect and apparent clinical improvement in lesions suggestive of erythematous candidiasis in palatar mucosa of rats. (AU) | |
| FAPESP's process: | 12/16805-5 - ANTIFUNGAL ACTIVITY OF PHENOLYIC ENRICHED FRACTION OF Buchenavia tomentosa AND SOME ISOLATED SUBSTANCES BEFORE AND AFTER ENCAPSULATION WITH BETA-CYCLODEXTRIN |
| Grantee: | Guilherme Rodrigues Teodoro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |