Genotypic characterization of Arcobacter spp. isolated from poultry meat sold in the city of São Paulo - SP

Author(s)

Maria Gabriela Xavier de Oliveira

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2016-01-13

Examining board members

Terezinha Knöbl; Andrea Micke Moreno; Eliana Scarcelli Pinheiro

• Advisor: Terezinha Knöbl

Abstract

Arcobacter spp. is a Gram negative microorganism that causes watery diarrhea and septicemia in humans. A. butzleri, A. cryaerophilus and A. skirrowii are pathogenic for humans. The aim of this study was to detect the presence of Arcobacter spp. in poultry meat from butcher shops in the city of São Paulo, checking the virulence genes and profile genotypic. A total of 300 chicken cuts were used for cultivation and isolation in broth and JM agar, under aerobic conditions, at 30 °C for 72 hours. Arcobacter colonies were selected and submitted to molecular detection by polymerase chain reaction (PCR), in order to determine species and virulence genes. Results show the presence of Arcobacter spp. in 18.3% (55/300) of poultry meat samples, identified as A. butzleri 63.6% (35/55) and A. cryaerophilus 36.3% (20/55) in chicken isolates. The virulence genes on Arcobacter spp. researched demonstrated positive these 100% (55/55) for ciaB and mviN, followed by cj134998,1% (54/55), pldA 94,4% (52/55), cadF 72.7% (40/55), tlyA 92,7% (51/55), hecA 49% (27/55), irgA 47,2% (26/55) and hecB 34,5% (19/55). These strains were submitted to AFLP generating two dendogramas. Nineteen profiles genotypins were obtained for A. butzleri and seventeen for A. cryaerophilus. The results of this research point to the presence of Arcobacter butzleri and A. cryaerophilus in the final stage of distribution of poultry meat to butcher shops. The lack of safety of food of animal origin, as well as the presence of virulent strains pose risks to Public Health, with special focus on cross contamination risks generated by uncooked food and utensils (AU)

• FAPESP's process: 14/06584-7 - Genotypic characterization of Arcobacter spp. isolated from poultry meat marketed in São Paulo-SP
• Grantee: Maria Gabriela Xavier de Oliveira
• Support Opportunities: Scholarships in Brazil - Master