Chronic, continuous and invariable body exposure to cortisol increases abdominal subcutaneous adipose tissue.

Author(s)

Patricia Pereira Nunes

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas
• Defense date: 24 mar. 2016

Examining board members

Fabio Bessa Lima; Nilton de Almeida Brito; Rui Curi

• Advisor: Fabio Bessa Lima

Abstract

Chronic use of high doses of glucocorticoids (GC) increases central adipose tissue (AT). This research aimed to verify the effects of continuous use of lower doses of GC on different TAs. Male Wistar rats, 10 weeks old, were divided into 2 groups: control (CON) and Cortisol (CORT), and were implanted with osmotic minipump, whereby CORT group received 0.6 mg/kg/d of cortisol and CON group received saline, for 6 weeks. The results show an increase in abdominal subcutaneous fat (SC) of CORT animals accompanied by: increased maximum activity of ATP citrate lyase and glucose 6-phosphate dehydrogenase enzymes; reduced expression of AMPK enzyme; increased expression of 11βHSD1 enzyme and circulating triglycerides. These data suggest that the intervention increased abdominal SC due to increased activity of lipogenic enzymes and a possible increase in the uptake of serum lipids through this tissue, which may have resulted from increased local GC concentration caused by increased expression of 11βHSD1. (AU)

• FAPESP's process: 13/24557-4 - Characteristics of differentiated lipogenic response in adipose tissue of rats subjected to excessive and prolonged stimulation with glucocorticoids
• Grantee: Patricia Pereira Nunes Traldi
• Support type: Scholarships in Brazil - Master