OMP29 Aggregatibacter actinomycetemcomitans: phylogenetic analysis, interaction with matrix proteins and response of epithelial cells.

Author(s)

Maike Paulino da Silva

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-04-27

Examining board members

Marcia Pinto Alves Mayer; Marinella Holzhausen Caldeira; Waldir Pereira Elias Junior; Rita de Cassia Cafe Ferreira; Luciana Saraiva

• Advisor: Marcia Pinto Alves Mayer

Abstract

OMP29 is one of the major outer membrane proteins of Aggregatibacter actinomycetemcomitans (Aa) and is associated with invasion into gingival epithelial cells (GEC). This study aimed to evaluate phylogenetically omp29 and omp29 paralogue (omp29par) in Aa strains; determine the interaction of OMP29 with extracellular matrix proteins and its effect on GEC by gene expression analysis and production of inflammatory mediators. Phylogenetic variations were observed for omp29 and omp29par as well as their promoters, and they were related to the serotypes. The recombinant protein OMP29his interacted with plasma and cellular fibronectin (p <0.05), but not to F30 and F45 domains, neither to collagens I, III, IV and V, fibrinogen, laminin and plasminogen. The interaction of Aa mutants defective in omp29 and/or omp29par (obtained by LoxP system/Cre) and OMP29his with CEG OBA-09 indicated that OMP29 regulates positively il-18 and negatively il-6r and il-8 (p <0.05). Data suggested that OMP29 is involved in bacterial evasion of the immune system. (AU)

• FAPESP's process: 11/18683-1 - Aggregatibacter actinomycetemcomitans: gene transcription profile of clinical isolates and the role of immune response against omp29 in the induction of experimental periodontitis in animal.
• Grantee: Maike Paulino da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate