Influence of TLR4 and TLR2 in behavioral and biochemical effects induced by intermittent fasting in knockout mice.

Author(s)

Andrea Rodrigues Vasconcelos

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-05-25

Examining board members

Cristoforo Scavone; Fernanda Marques da Cunha; José Donato Júnior; Renata Gorjão; Sérgio Gomes da Silva

• Advisor: Cristoforo Scavone; Elisa Mitiko Kawamoto Iwashe

Abstract

Intermittent fasting (IF) stimulates the body\'s defense mechanisms, making it more resistant to toxic stimuli. IF seems to act by mechanisms associated with cell survival, autophagy, inflammation and enhancing oxidative stress resistance, thereby involving the modulation of transcription factors. However, little is known about the involvement of TLR4 and TLR2 on IF effects. The present work investigated the influence of TLR2 and TLR4 on IF effects on memory and on signaling mechanisms associated with the transcription factors NF-κB, NRF2 and FOXO in TLR2 KO or TLR4 KO mice. The results suggest that TLR4 and TLR2 participate in the effects of IF on oxidative stress levels, peripheral biomarkers, and on NF-κB, CREB, AP1 and NRF2, as well as proteins modulated by these transcription factors such as BDNF, HO1, antioxidant enzymes, chaperones and cytokines. These results allow a better understanding of physiological processes that aim at developing new pharmacological interventions to promote longevity, healthy aging, and the treatment of neurodegenerative disorders. (AU)

• FAPESP's process: 11/12255-8 - Influence of TLR4 receptors in behavioral and neurochemical alterations caused by intermittent fasting in C3H/HeJ mice
• Grantee: Andrea Rodrigues Vasconcelos
• Support Opportunities: Scholarships in Brazil - Doctorate