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| Author(s): |
Natália Carrillo Gaeta
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2016-08-08 |
| Examining board members: |
Lilian Gregory;
Alessandra Figueiredo de Castro Nassar;
Maria Claudia Araripe Sucupira
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| Advisor: | Lilian Gregory |
| Abstract | |
Bovine respiratory disease complex is one of the major problems observed in feedlot and grazing cattle, causing economic losses due to high morbidity and mortality rates. The aim of this study was to determine the prevalence of M. bovis, M. díspar, M. mycoides subsp. mycoides SC, P. multocida, M. haemolytica, Bovine Parainfluenza type-3 (bPI-3) and Influenza vírus D (IVD) and the prevalence of antibodies against Bovine Viral Diarrhea Vírus (BVDV), Bovine Herpesvírus type -1 (BoHV1) and Bovine Respiratory Syncytial Vírus (VRSB) in healthy and pneumonic calves raised in settlements located in Caiuá, Presidente Epitácio e Mirante de Paranapanema São Paulo State. Moreover, we aimed to evaluate the association between those microorganisms and antibodies and the presence of bronchopneumonia and the symptoms observed during the physical examination. We studied 141 males and females calves that were classified as healthy and pneumonic calves. We collected tracheobronchial lavage samples and total blood to obtain serum samples. Isolation and biochemical/ molecular identification were performed in order to detect M. bovis, M. díspar, M. mycoides subsp. mycoides SC, P. multocida, M. haemolytica, Bovine Parainfluenza type-3 and Influenzavírus D. Serological survey was performed to detect antibodies aginst Bovine Viral Diarrhea Vírus (BVDV), Bovine Herpesvírus type -1 (BoHV1) and Bovine Respiratory Syncytial Vírus (VRSB). There was not any isolation of P. multocida and M. haemolytica. Bacillus sp., Staphylococcus intermedius and Non-fermenter Gram-negative bacteria were higher prevalent compared to the other aerobic bacteria isolated. M. díspar was the only mycoplasma species identified by the primers used. It is necessary the identification of other mycoplasma species that could be related to the cases of bronchopneumonia in cattle. Bovine Parainfluenza type-3 and Influenza vírus D were not identified. Antibodies were detected in all municipalities. The prevalence of BoHV, BVDV e VRSB was 31.7%, 24.6 and 38.8%, respectively. The association between health status and microbial/ serological findings was not observed. Enterobacterias were associated to “mild dehydration” (p=0.033/OR=11.25; IC: 1.809-41.834). Associations between Mollicutes and “serous nasal discharge” (p=0.03) was also detected. In conclusion, S. intermedius was associated to “nasal air flow” (p=0.097). In conclusion bovine bronchopneumonia is multi-factorial disease. It is necessary, though, physical examination, microbiology and management and environment feature results (AU) | |
| FAPESP's process: | 14/03188-3 - Clinical evaluation of bronchopneumonia of calves in the settlements in the region of Presidente Epitácio and Presidente Venceslau |
| Grantee: | Natália Carrillo Gaeta |
| Support Opportunities: | Scholarships in Brazil - Master |