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Role of MuRF1 and MuRF2 on functional and structural aspects of myoblasts and fibroblasts skeletal muscle cells.

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João Guilherme de Oliveira Silvestre
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Anselmo Sigari Moriscot; Raquel Agnelli Mesquita Ferrari; Claudimara Ferini Pacicco Lotfi; Maria Tereza Nunes; Edmar Zanoteli
Advisor: Anselmo Sigari Moriscot

The E3 ligases MuRF1 and MuRF2 has been proposed to act as a linker for myofibril machinery, also by acting as an Atrogene during muscle wasting. Our aim was to verify the role of MuRF1 and MuRF2 during the myogenic differentiation of skeletal muscle and its role on skeletal muscle fibroblasts. We used MuRF1 and MuRF2 knockout mice and analyzed the regenerative process. Using in vitro analyzes, we silenced MuRF1 and MuRF2 expression by siRNA. Our results suggest that knockout mice had an important impairment on skeletal muscle regeneration, showing positive staining to white adipocytes. Moreover, siRNA on myogenic primary cultures showed impaired myotube formation and increase the expression of adipogenic markers. Another interesting finding was that skeletal muscle fibroblasts can express MuRF1 and MuRF2, and its silencing by siRNA impairs the migration capacity of fibroblasts. These results demonstrate the importance of MuRF1 and MuRF2 during myogenic differentiation of skeletal muscle and an important role at intracellular coordination of stress fiber formation of skeletal muscle fibroblasts. (AU)

FAPESP's process: 12/13315-7 - Role of MuRF1 and MuRF2 in skeletal muscle myogenic cell differentiation
Grantee:João Guilherme de Oliveira Silvestre
Support Opportunities: Scholarships in Brazil - Doctorate