Cloning, purification and characterization of recombinant antigenic protein of Mycoplasma agalactiae.

Author(s)

Maysa Santos Barbosa

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-09-26

Examining board members

Jorge Timenetsky; Andrea Balan Fernandes; Lilian Gregory; Alexandre Lourenço

• Advisor: Jorge Timenetsky

Abstract

The Contagious agalactia is a notifiable disease that causes severe economic losses to sheep and goats worldwide. Despite its impact on animal production, little is known about the virulence factors and pathogenicity of M. agalactiae (main etiological agent). Thus, the present study identified, purified and characterized antigenic proteins of M. agalactiae. Therefore, four surface proteins with antigenic potential (WP_011949419.1, WP_011949418.1 (P40), WP_011949336.1, WP_011949770.1) were selected. These proteins were expressed in Escherichia coli and purified on nickel column. The purified proteins were assayed for antigenicity by Western blotting using goat sera naturally infected with M. agalactiae. All expressed proteins were immunoreactive with sera from naturally infected goats, demonstrating that the proteins used in this study are possibly antigenics and it have acessible epitopes. (AU)

• FAPESP's process: 14/09425-7 - Cloning, purification and characterization of recombinant antigenic proteins derived from Mycoplasma agalactiae
• Grantee: Maysa Santos Barbosa
• Support Opportunities: Scholarships in Brazil - Master