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Micromorphological description and effects of benzopyrene in liver and blood cells of species Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)

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Author(s):
Lara Zácari Fanali
Total Authors: 1
Document type: Master's Dissertation
Press: São José do Rio Preto. 2016-03-22.
Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto
Defense date:
Advisor: Classius de Oliveira; Cláudia Regina Bonini Domingos
Abstract

Amphibians are influenced by several pollutants that may affect their life cycle. Benzo[α]pyrene (BaP) is a bioaccumulative contaminant found in the atmosphere, water and soil. In order to measure how this pollutant acts in the species Physalaemus cuvieri and Leptodactylus fuscus, specific biomarkers were utilized, such as hepatic melanomacrophages, which displays detoxification functions; mast cells, which are protective cells during inflammatory response; micronuclei (MN), DNA fragments resulting from the exposure to toxic agents; white blood cells, due to leukocytes patterns alterations indicating that the organism is not in its normal conditions. Initially, the description of the species liver (6 animals of each species) was performed by means of light and transmission electron microscopy. It was observed that the hepatic tissue is very similar between species. For the BaP study, 36 animals of each species were used, divided in 6 treatments. The treated ones received a subcutaneous injection (2 mg/kg) of BaP, diluted in mineral oil, while control animals received only mineral oil. The following processes were performed for blood and light microscopy analysis: with 7d of treatment, melanin area were reduced in both species, followed by an increased in 13d for the species L. fuscus. This decline may be related to the cytotoxic effect of the compound or the organism physiological adjustment. Mast cells displayed increased density in both species and in all treated groups. This response is linked to the cells’ protection function on inflammatory responses. No changes in MN frequency were found for both species, probably because most damaged cells underwent apoptosis or lost the capacity of initializing mitosis. After 13d of treatment, an increase in neutrophils was observed, given its function in inflammatory responses, such as a decrease in eosinophils, due to immune function alteration. (AU)

FAPESP's process: 14/07466-8 - Effects of the benzo[alfa]pyrene in liver and blood of the species Physalaemus cuvieri and Leptodactylus fuscus (Anura: Leptodactylidae)
Grantee:Lara Zácari Fanali
Support Opportunities: Scholarships in Brazil - Master