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| Author(s): |
Luciana Tiemi Inagaki
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2012-02-27 |
| Examining board members: |
Regina Maria Puppin Rontani;
Cássia Cilene Dezan Garbelini;
Fernanda Miori Pascon
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| Advisor: | Regina Maria Puppin Rontani |
| Abstract | |
This dissertation was divided into two chapters: Chapter 1 aimed to evaluate the antimicrobial activity of cured and uncured resin blends though measurement of inhibition zone using agar diffusion (Pour Plate), Minimal Inhibiting Concentration (MIC) and Minimum Bactericidal Concentration (MBC). For microbiological assays, that were performed in triplicate, the 0.12% chlorhexidine digluconate solution was used as reference standard antimicrobial and infiltrant Icon® (DMG) was used as commercial control group. Streptococcus mutans UA159 and Lactobacillus acidophilus LYO50DCU-S strains were selected for all assays. Chapter 2 aimed to determine DC and Knoop hardness of mixtures having Icon® as commercial control group. Mixtures were set as follow: TEGDMA, TEGDMA/0.1% CHX, TEGDMA/0.2% CHX, TEGDMA/UDMA, TEGDMA/UDMA/0.1% CHX, TEGDMA/UDMA/0.2% CHX, TEGDMA/BisEMA, TEGDMA/BisEMA/0.1% CHX and TEGDMA/BisEMA/0.2% CHX. Data obtained from all mixtures and Icon® were submitted to one-way ANOVA, followed by Tukey test, and in order to compare the groups t-test was used (p<0.05). The MIC and MBC tests showed that the mixtures demonstrated antibacterial activity in low concentrations of CHX against both of strains. Analyzing antibacterial activity against S. mutans before light curing process, the most of blends provided larger inhibition zones than Icon®, and for TEGDMA/BisEMA/0.1% CHX and TEGDMA/BisEMA/0.2% CHX the CHX concentration was significant factor to increase the inhibition zones. After light curing, the mixture TEGDMA/UDMA/0.1% CHX showed the highest inhibition zone against S. mutans. Analyzing antibacterial activity against L. acidophilus before light curing, the addition of CHX to the blends, regardless concentration, provided inhibition zones and TEGDMA/0.2% CHX and TEGDMA/UDMA/0.1% CHX showed the highest antibacterial effects. After light curing, no significant difference between all experimental blends, including Icon®, was observed. When the inhibition zones of uncured and cured blends were compared, the major of uncured blends demonstrate grater antimicrobial activity to both strains. The addition of CHX didn't reduce the DC of experimental infiltrants blends, but CHX had positive influence for TEGDMA neat monomer, increasing DC. TEGDMA/UDMA, TEGDMA/UDMA/0.1% CHX and TEGDMA/UDMA/0.2% CHX showed the highest DC than other mixtures, while TEGDMA/BisEMA, TEGDMA/BisEMA/0.1% CHX and TEGDMA/BisEMA/0.2% CHX the lowest DC. All mixtures showed significant lower values of DC than commercial infiltrant. Concerning hardness, TEGDMA/UDMA and TEGDMA/UDMA/0.2% CHX showed the highest Knoop hardness values. The addition of CHX didn't change surface hardness of mixtures. When Knoop hardness values of nine mixtures were compared with Icon®, all mixtures showed significant higher values than commercial infiltrant. Thus, among the experimental resin mixtures evaluated, TEGDMA/UDMA/0.1% CHX showed the best results to DC and to antimicrobial effect after polymerization (AU) | |
| FAPESP's process: | 10/01010-1 - Analysis of physical, mechanical and antibacterial properties of infiltrant materials related the composition: base monomers, diluents and antimicrobial |
| Grantee: | Luciana Tiemi Inagaki |
| Support Opportunities: | Scholarships in Brazil - Master |