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Modification of temporary resilient liners for dentures with antimicrobial agents: in vitro and in vivo studies


The incorporation of antifungal agents / antimicrobials in temporary denture relines has proved to be a viable alternative for the treatment of denture stomatitis. This treatment has the advantage of breaking the re-infection of supporting tissue via denture-fitting surface as it eliminates the contact of the prosthetic biofilm with the infected tissues, by acting upon them through the incorporated drugs, which are released gradually over the clinical life of these materials. Previous studies demonstrated that up to 14 days, the antifungal nystatin and antimicrobial chlorhexidine diacetate added to temporary relines (Trusoft e Softone) were the drugs that showed the minimum inhibitory concentrations (MICs) for biofilm of C. albicans. Furthermore, it was observed that the addition of these drugs did not result in deleterious effects on the Shore A hardness, surface roughness and tensile strength of the materials. Despite of these favorable results, information about the effects of the addition of antimicrobial agents in its MCIs on other properties of the resilient relines is lacking. This present study will evaluate in vitro the effect of the addition of the MICs of antimicrobial agents used for denture stomatitis' treatment upon water sorption, solubility and porosity of temporary resilient lining materials. To calculate the water sorption values and solubility, specimens (50 X 0.5 mm) will be dried, immersed in water for 24 h, 7 e 14 days, weighed, dried, and weighed again. For porosity analysis, specimens (45 X 60 X 2 mm) modified or not by addition of the drugs will be produced and the images of porous displayed in a scanning electron microscopy (x100) after 24h, 7 and 14 days. SEM microphotographs will be analyzed using Leica Qwin image analyzer software to determine the number and total area of porosities. For the in vitro studies, the experimental results will be compared with the controls and the data analyzed statistically (±=5%). The material-drug combinations that cause the least harmful effects to the properties of materials will be tested for biocompatibility. Histopathological analysis of the palatal mucosa of rats will be performed after 7 and 14 days of wearing acrylic resin splint covering the palate and relined with the resilient material, modified or not by the addition of drug. Fifty rats will be divided into five groups: Negative control (NC): absence of palatal splint; Overall Control (OC): palatal splint without relining, Positive Control (PC): palatal splint relined with resilient material without addition of drug; Material-drug 1 (MD1): palatal splint relined containing MIC of drug from combination 1; Material-drug 2 (MD2): palatal splint relined with resilient material containing MIC from combination 2. For histopathological analysis, half of the sample from each group (n=5) will be sacrificed after 7 days and half after 14 days. Images will be displayed in an optical microscope (40x), and quantitative analysis performed by computerized planimetry. Histopathological parameters will be compared with the controls and the data analyzed statistically (±= 5%). (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ADELAIDA SÁNCHEZ-ALIAGA; PAULO VITOR FARAGO; MILTON DOMINGOS MICHÉL; CAROLINA YOSHI CAMPOS SUGIO; KARIN HERMANA NEPPELENBROEK; VANESSA MIGLIORINI URBAN. Surface morphology and in vitro leachability of soft liners modified by the incorporation of antifungals for denture stomatitis treatment. Journal of Applied Oral Science, v. 28, p. -, 2020. Web of Science Citations: 0.
MACIEL, JANAINA GOMES; CAMPOS SUGIO, CAROLINA YOSHI; CHAVES, GIULIANA DE CAMPOS; FALCAO PROCOPIO, ANDREA LEMOS; URBAN, VANESSA MIGLIORINI; NEPPELENBROEK, KARIN HERMANA. Determining acceptable limits for water sorption and solubility of interim denture resilient liners. JOURNAL OF PROSTHETIC DENTISTRY, v. 121, n. 2, p. 311-316, FEB 2019. Web of Science Citations: 0.
HOTTA, JULIANA; GARLET, GUSTAVO POMPERMEIER; CESTARI, TANIA MARY; MELLO LIMA, JOZELY FRANCISCA; PORTO, VINICIUS CARVALHO; URBAN, VANESSA MIGLIORINI; NEPPELENBROEK, KARIN HERMANA. In vivo biocompatibility of an interim denture resilient liner containing antifungal drugs. JOURNAL OF PROSTHETIC DENTISTRY, v. 121, n. 1, p. 135-142, JAN 2019. Web of Science Citations: 1.
PROCOPIO, A. L. F.; DA SILVA, R. A.; MACIEL, J. G.; SUGIO, C. Y. C.; SOARES, S.; URBAN, V. M.; NEPPELENBROEK, K. H. Antimicrobial and cytotoxic effects of denture base acrylic resin impregnated with cleaning agents after long-term immersion. TOXICOLOGY IN VITRO, v. 52, p. 8-13, OCT 2018. Web of Science Citations: 1.
HOTTA, JULIANA; CRAL, WILSON GUSTAVO; SAKIMA, VINICIUS TATSUYUJI; LARA, VANESSA SOARES; URBAN, VANESSA MIGLIORINI; NEPPELENBROEK, KARIN HERMANA. Intraoral Device for Optimal Antifungal Delivery in a Rat Model. CURRENT DRUG DELIVERY, v. 14, n. 5, p. 658-667, 2017. Web of Science Citations: 1.
JOZELY FRANCISCA MELLO LIMA; JANAÍNA GOMES MACIEL; JULIANA HOTTA; ANA CAROLINA PERO VIZOTO; HEITOR MARQUES HONÓRIO; VANESSA MIGLIORINI URBAN; KARIN HERMANA NEPPELENBROEK. Porosity of temporary denture soft liners containing antifungal agents. Journal of Applied Oral Science, v. 24, n. 5, p. 453-461, Out. 2016. Web of Science Citations: 3.
URBAN, VANESSA M.; LIMA, THIAGO F.; BUENO, MIRIAN G.; GIANNINI, MARCELO; ARIOLI FILHO, JOAO N.; DE ALMEIDA, ANA LUCIA P. F.; NEPPELENBROEK, KARIN H. Effect of the Addition of Antimicrobial Agents on Shore A Hardness and Roughness of Soft Lining Materials. JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY, v. 24, n. 3, p. 207-214, APR 2015. Web of Science Citations: 9.

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