Correlation of the Runx1, ETV5, MMP-2 and MMP-9 expression in oral leukoplakia and oral squamous cell carcinoma

Author(s)

Marcondes Sena Filho

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2016-01-29

Examining board members

Jacks Jorge Junior; Claudio Maranhão Pereira; Aline Carvalho Batista; Pablo Agustin Vargas; Oslei Paes de Almeida

• Advisor: Jacks Jorge Junior

Abstract

Oral squamous cell carcinoma (OSCC) is the most common malignancy of the head and neck region. The mucosal changes that precede the occurrence of OSCC are of great interest in this context, in which the oral leukoplakia (OL) is a subject of several studies. Runx1 is a transcription factor involved in physiological and pathologic events of hematopoiesis, but its functions in solid tumors is still poorly understood. Some studies have suggested a possible interaction of Runx1 with others transcription factors, as ETV5, stimulating the production of matrix metalloproteinases (MMP) 2 and 9 in some solid malignancies, as endometrioid carcinoma and chondrosarcoma. Through immunohistochemistry, immunofluorescence, Western blot and zymography, this study evaluated and correlated the expression of Runx1 and ETV5 with the expression of MMP-2 and MMP-9, epithelial basal membrane integrity and cellular proliferation index in OL, OSCC and normal oral mucosa. The results demonstrated that the expression of Runx1 and ETV5 are correlated with high expression of MMPs 2 and 9, disruption of epithelial basement membrane and high proliferation index in OL with severe epithelial dysplasia and OSCC, which suggest that Runx1 and ETV5 play an important role in the oral squamous cell carcinoma development (AU)

• FAPESP's process: 13/15955-6 - Correlation of the transcription factors Runx1 and ETV5 with the expression and activity of metalloproteinases 2 and 9 in oral potentially malignant lesions and squamous cell carcinomas
• Grantee: Marcondes Sena Filho
• Support Opportunities: Scholarships in Brazil - Doctorate