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| Author(s): |
Camila Dalben Madeira Campos
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2016-02-03 |
| Examining board members: |
José Alberto Fracassi da Silva;
Frank Nelson Crespilho;
Carolina Palma Naveira Cotta;
Ivo Milton Raimundo Júnior;
Jarbas José Rodrigues Rohwedder
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| Advisor: | José Alberto Fracassi da Silva; Felix Guillermo Reyes Reyes |
| Abstract | |
In this project we evaluated different possibilities for the development of a Lab on a chip able to detect and quantify amino acids in complex matrices, particularly foodstuff. We aimed an analysis specially focusing monosodium glutamate ¿ which is a neurotransmitter and essential amino acid as well as the main responsible for Umami taste in food. We searched for a practical, economical, efficient and fast manner, suitable for small volumes of sample, aiming to solve the demands in production and sanitary control. To this purpose, we developed both the separation process, based on capillary electrophoresis and the sample cleaning steps, miniaturized them and coupled both on a single device. With this strategy, we achieved detection limits around 0.3 to 0.5 mmol L-1, suitable to most food samples. An additional chapter was dedicated to other strategies studied with the same objective, that were not so suitable to our context, but that would be an interesting starting point for other applications, situations and products (AU) | |
| FAPESP's process: | 11/02477-3 - Development of a Lab-on-a-chip for glutamate analysis in Foodstuffs |
| Grantee: | Camila Dalben Madeira Campos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |