Full text
| |
| Author(s): |
Paula Fernanda de Lima
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2017-01-10. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu |
| Defense date: | 2016-11-04 |
| Advisor: | José Buratini Junior |
| Abstract | |
The oocyte is responsible for regulating mechanisms of cumulus oocyte complex (COC) maturation mainly through secretion of paracrine factors. Including oocyte secreted factors (OSF) are the fibroblast growth factor 10 (FGF10), and two TGF beta factors most studied, bone morphogenetic protein 15 (BMP15) differentiation and growth factor 9 (GDF9), and they are associated with the oocyte development of competence. This study investigated the regulation of oocyte cumulus cells on two current scenarios in vitro maturation in the presence of follicle stimulating hormone (FSH) or ampirregulina (AREG). The absence of oocyte affects the expansion of the cumulus cells and also the glucose consumption and lactate production and addition of denuded oocytes reverses the effect of the expansion oocectomia but not glucose consumption or lactate production. The oocectomia also affects gene expression by reducing the abundance of mRNA hialurona synthetase 2 HAS2 and raising synthase prostaglandin expression 2 (PTGS2), pentraxin 3 (PTX3) and inducing protein of the tumor necrosis factor 6 (TNFAIP6) in the presence of FSH and AREG. The addition of denuded oocytes reverses the effect of AREG on oocectomia mRNA expression at 4 hours of culture, HAS2 and PTGS2 to 22 hours of cultivation only when complexes were grown in the presence of AREG. Additionally it investigated the isolated FGF10 action on the differentiation of cumulus cells. FGF10 reverses the effect of oocectomia on the expansion of the cells and the expression of HAS2 and GFPT1apenas to 22 hours in cumulus but not glucose consumption or lactate production, which shows a delayed effect of FGF10 necessitating the presence of other OSF to soon effect as seen previously in the literature. Complementary to the results, it appears that the cumulus cells have an intracumulus control Smad2-activation by the presence of GDF9 and BMP15 in cumulus cells. In summary the oocyte secreted factors regulate the functions of the cumulus cells and the mRNA expression of important genes during maturation of the COC and these factors may differentially regulate cumulus cells in the presence of FSH or AREG. (AU) | |
| FAPESP's process: | 12/15820-0 - Fibroblast Growth factors (FGFs)and bone morphogenetic protein 15 (BMP15) on in the diferentiation of bovine cumulus cells. |
| Grantee: | Paula Fernanda de Lima |
| Support Opportunities: | Scholarships in Brazil - Doctorate |