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| Author(s): |
Ligia Maria Neira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2017-01-30. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2016-11-30 |
| Advisor: | Dalton José Carneiro |
| Abstract | |
In Aquaculture, to be sustainable should use of the waste to produce fish silages. Three experiments were conducted to study the feasibility of fractionation protein from tilapia waste hydrolysate through evaluations of the hydrolysis processes of protein and the efficient use of their polypeptides in practical diets on performance, nutrition and intestinal morphology. The experiment 1, aimed to produce and evaluate the silages of Nile tilapia filleting waste. Two types of raw materials, viscera or total residues were used (head, spine, fins and viscera), two processes of hydrolysis, acid and fermented with three methodologies to interrupt the processes, heating, freezing or neutralization. The experimental results showed that the hydrolysis took place at different times for different raw materials and hydrolysis processes. The silage production was viable and showed favorable characteristics for this product use in fish diets. The second experiment was carried out to determinate the apparent digestibility of crude protein (ADCCP), gross energy (ADCGE), lysine (ADCLys) and methionine (ADCMet) of fish silages with different time of hydrolysis in diets for Nile tilapia juvenile. 720 juveniles were used (9.36 ± 0.74 g). 12 test diets were formulated, constituted 69.5% of a reference diet, 0.5% chromium III oxide and 30% of an acid or fermented silage, produced with viscera or total residues at different times of hydrolysis (SVA 24, 72 and 144 hours SVF 24, 48 and 120 hours, and STA STF 24, 96 and 192 hours of hydrolysis). The ADC mean of protein, lysine and methionine silages ranged from 91.75 to 95.80%. The lowest ADC values were determined for crude energy and ranged from 79.30% to 83.62%. These results showed that only total waste silage have high levels of CP and ADCCP. The times 96 and 192 hours of hydrolysis were selected to the acid and fermented, respectively, for use as ingredients in practical diets for Nile tilapia. The third experiment was aimed to evaluate the performance and nutritional efficiency of Nile tilapia juvenile fed with eight different diets containing tilapia meal and partial or total replacement for acid (SA) or fermented (SF) fish silage, soybean protein isolate (SPI) or free amino acid (FAA). 640 juveniles were used (7.18 ± 5.43 g). The results showed that the fish fed diets containing TM, partial replacement (SA50, ST50 and IPS50) and total replacement by SA showed the best results of feed efficiency, and their growth was significantly higher when compared to fish that received the diets containing SF and FAA. These results show that the substitution of FT by hydrolyzed was possible (AU) | |
| FAPESP's process: | 13/16773-9 - Production of polypeptide of protein hidrolysates and their efficiency in nutrition of juveniles of Nile Tilapia |
| Grantee: | Ligia Maria Neira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |