Tissue response evaluation and mineralization ability of Biodentine and White MTA cements

Author(s)

Diego Valentim

• Document type: Doctoral Thesis
• Press: Araçatuba. 2017-04-19.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2017-02-21
• Advisor: Eloi Dezan Junior

Abstract

The aim of the study was to evaluate the tissue response and the mineralization capacity of the endodontic materials, Biodentine®, White MTA Angelus®, when compared with calcium hydroxide. Forty-eight Wistar rats were used; 24 for subcutaneous analysis and 24 underwent pulpotomy. After 7, 15, 30 and 60 days (subcutaneous) the animals were euthanized and histological and immunohistochemical processing were performed (Fibronectin and Tenascin). After 7 and 15 days (pulpotomy) the pieces were submitted to histological and immunohistochemical processing (Fibronectin and Tenascin) to evaluate the formation of the hard tissue bridge and tissue response of the pulp. Data from both subcutaneous and pulpotomy were submitted to the Kruskal Wallis and Dunn test (p<0.05). Statistical analysis showed that in the subcutaneous tissue at 15 days Biodentine® generated a lower inflammatory response than the glass ionomer and Ca(OH)2 (p<0,05),while the MTA presented no statistical difference (p>0,05). In the pulp at 7 days, the MTA and the calcium hydroxide had a higher continuity of the hard tissue bridge than the glass ionomer (p<0,05) and Biodentine presented better morphological features than the glass ionomer (p<0,05). At 15 days the MTA and Biodentine showed a complete hard tissue bridge (p<0,05); For immunostaining, there was no statistical difference in the subcutaneous tissue; for pulpotomy, Biodentine obtained higher immunostaining than the glass ionomer for both Fibronectin and Tenascin. The Biodentine®, White MTA Angelus and Calcium Hydroxide presented the ability to induce mineralization in accordance with the methodologies applied in this study. (AU)

• FAPESP's process: 14/02327-0 - Evaluation of tissue response and the mineralization ability of Biodentine and White MTA sealers
• Grantee: Diego Valentim
• Support Opportunities: Scholarships in Brazil - Doctorate