Production of Cryptosporidium parvum recombinant proteins aiming the development of diagnostic methods

Author(s)

Milena Sato de Souza

• Document type: Doctoral Thesis
• Press: Araçatuba. 2017-08-24.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba
• Defense date: 2017-08-15
• Advisor: Marcelo Vasconcelos Meireles

Abstract

Cryptosporidium spp. are protozoan parasites that infect mainly the epithelial cells of the digestive tract in several animals including humans. This protozoan causes enteric disease, which may be a self-limiting diarrhea in healthy individuals, or even fatal illness in immunodeficient patients. Cryptosporidium parvum is the leading cause of infection in mammals and of zoonotic cryptosporidiosis. The development of new methods for detection, prevention, treatment and control strategies for cryptosporidiosis in humans are increasingly required. Antibodies and recombinant proteins have the potential for the development of drugs, vaccines or could be used to develop more affordable diagnostic techniques with high sensitivity and specificity. In this study, the main goal was the expression of a recombinant CP41 protein from C. parvum in Escherichia coli and preparation of antiserum specific for recombinant CP41 (rCP41). The research effort is related to detection of Cryptosporidium oocysts by immunofluorescence. (AU)

• FAPESP's process: 13/24082-6 - Expression and purification of Cryptosporidium parvum COWP5 recombinant protein aiming the production of polyclonal IgY in chickens for diagnostic purpose
• Grantee: Milena Sato de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate