Advanced search
Start date

Lung biopsy via paraxiphoid transdiafragmatic toracoscopy with hand tied loop through two portals in rabbits

Full text
Monica Carolina Nery Wittmaack
Total Authors: 1
Document type: Master's Dissertation
Press: Jaboticabal. 2017-08-29.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
Defense date:
Advisor: Paola Castro Moraes; Pedro Paulo Maia Teixeira

The aim of this study was suggest minimally invasive thoracoscopic technique using a biportal approach to perform lung biopsy in animals weighing less than 5 kg, moreover, it is intended to describe and evaluate the hand tied loop technique for low-weight animals. So ten rabbits were used as an experimental model, it was described thoracoscopy technique through two portals, with paraxiphoid transdiaphragmatic approach for the first portal, and intercostal approach for the second portal. In this study insufflation of the thoracic cavity was not performed. During surgery no complications associated with the impossibility of sliding and tightening knot or as escape knot after lung biopsy. In all animals, distal of the ligature were observed lung tissue congestion due to occlusion of vessels and airways. The miniloop system was considered effective for air leakage and hemorrhage after lung biopsy. The total surgical time from skin incision to suture was 41.4 ± 14.5 minutes. Tissue sample sizes obtained averaging 1x0,64x0,45cm and were considered satisfactory second histopathological evaluation. Preoperative and postoperative radiographs were performed and compared in order to verify the absence of pneumothorax and hemothorax. The necopsy was performed on the 15th day after surgery. Adhesions between biopsy site and mediastinal membrane and adhesion between biopsy site and parietal pleura were findings in five animals. The hand tied loop were properly positioned and a margin of the pulmonary parenchyma 2 to 4 mm distal to the ligature was left to avoid complication as slipping knot. The proposed technique allowed access to the pleural cavity with minimal trauma to the thoracic structures and made possible the development of lung biopsy by extracorporeal knot with low occurrence of trans and postoperative complications. (AU)

FAPESP's process: 15/19965-1 - Lung biopsy via paraxiphoid transdiafragmatic toracoscopy with hand tied loop through two portals in rabbits: experimental study
Grantee:Monica Carolina Nery Wittmaack
Support Opportunities: Scholarships in Brazil - Master