Cryopreservation of human semen in Yolk Buffer test medium or synthetic medium supplemented with phospholipid and antioxidant: controlled clinical trial

Author(s)

Aline Bomfim Silva

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC)
• Defense date: 2017-08-11

Examining board members

Rosana Maria dos Reis; Carlos Augusto Fernandes Molina; Fábio Firmbach Pasqualotto

• Advisor: Rosana Maria dos Reis

Abstract

OBJECTIVES: To evaluate the efficacy of a synthetic cryopreservation medium for human semen containing phospholipids and antioxidants (ANTIOX-PC, Invitra / Supera Parque de Inovação e Tecnologia de Ribeirão Preto) compared to conventional egg yolk medium (TEST-yolk buffer, Irvine Scientific), measured by the in vitro parameters of progressive sperm motility and sperm DNA fragmentation index. METHODS: Non-inferiority clinical trial. 63 men (July 2015 to October 2016), aged 18 to 50 years, seminal sample with volume >= 1,5 mL, spermatozoa >= 15 x 106/mL and progressive motility >= 32% were recruited. The semen was divided into two aliquots with equal volumes, randomly randomized in the study (ANTIOX-PC) and control (TEST-yolk buffer) groups to receive cryopreservation media. The evaluation of the outcomes was blind to group assignment, verified before freezing and after thawing. The data were compared by the t-test paired by SAS version 9.3; ? = 0.05. RESULTS: Progressive motility (p=0.83) and DNA fragmentation index (p=0.32) analyzed in the semen samples frozen with medium A (ANTIOX-PL) showed no significant difference in relation to those frozen with the medium B (TEST-yolk buffer). The concentration (p=0.02), the total concentration (p=0.02), the percentage of immobile spermatozoa (p<0.01) and vitality (p<0.01) were higher in the samples frozen with medium B TEST-yolk buffer). Nonprogressive motility (p<0.01) and total motility (p <0.01) were higher in samples frozen with medium A (ANTIOX-PC). And the morphology (p=0.07) showed no significant difference between the cryopreservation media. CONCLUSIONS: The new formulation proposed in this research project, ANTIOX-PC, was not inferior to the conventional medium, TEST-yolk buffer, in relation to the primary endpoints analyzed, sperm motile spermatozoa and sperm DNA fragmentation index. (AU)

• FAPESP's process: 14/13477-2 - Cryopreservation of human semen in test yolk buffer medium or synthetic medium supplemented with phospholipid and antioxidant: a controlled clinical trial
• Grantee: Aline Bomfim Silva
• Support Opportunities: Scholarships in Brazil - Master